水产学报2017，Vol.41Issue(11)：1710-1718,9.DOI:10.11964/jfc.20170410808
凡纳滨对虾C型凝集素(LvLc1)的特征和活性分析
Characterization and bioactivity analysis of a novel c-type lectin (LvLc1) from Pacific white shrimp (Litopenaeus vannamei)
摘要
Abstract
As a family of carbohydrate binding proteins with Ca2+, C-type lectins play important roles in the first line of innate immune defense.In this research, a novel C-type lectin gene (LvLc1, GenBank Accession Number:KY937940) was cloned from Litopenaeus vannamei according to the data of shrimp transcriptome in our lab.The full-length cDNA consists of 1251 bp with an 891 bp open reading frame, encoding 296 amino acids.The deduced amino acid sequence contains a putative signal peptide of 19 amino acids.It also contains one carbohydrate recognition domains/C-type lectin-like domains (CRD).The potential carbohydrate-binding motif (QPD) presented in the CRD ofLvLc1 may support its ability to bind galactose-type sugars.The deduced amino acid sequence of LvLc1 showed high identity with mannose-binding lectins of arthropod Procambarus clarkii and Pacifastacus leniusculus.It could be deduced thatLvLc1 is a novel member of C-type lectin superfamily.The recombinant target protein (rLvLc1) was expressed by prokaryotic expression system.The LC-ESI-MS analysis showed that the peptide fragments of rLvLc1 were identical with the corresponding sequence of L.vannamei C-type lectin.rLvLc1 had agglutinating activity against main pathogens (G+, G– and fungi) in aquaculture in a calcium-dependent manner.The agglutinating activity can be inhibited by multiple carbohydrates, such as galactose, mannose and lipopolysaccharide.These results suggest thatLvLc1, as a Ca2+ dependent carbohydrate-recognition protein, is one of the important PRRs.It might play a crucial role in the innate immunity of the shrimp and it is expected to be applied to disease control.关键词
凡纳滨对虾/C型凝集素/糖识别结构域/重组表达/凝集/糖识别活性Key words
Litopenaeus vannamei/C-type lectin/carbohydrate recognition domain (CRD)/recombinant expression/agglutinating/carbohydrate binding activity分类
农业科技引用本文复制引用
李俊烨,宋巧珍,张亦陈,刘逸尘,耿绪云,孙金生..凡纳滨对虾C型凝集素(LvLc1)的特征和活性分析[J].水产学报,2017,41(11):1710-1718,9.基金项目
国家自然科学基金(31472299) （31472299）
天津市应用基础与前沿技术研究计划(15JCZDJC33800) （15JCZDJC33800）
天津市水产生态及养殖重点实验室开放基金(TJAE2015005) ,"十二五"农村领域国家科技支撑计划(2011BAD13B04) National Natural Science Foundation of China (31472299) （TJAE2015005）
Natural Science Foundation of Tianjin (15JCZDJC33800) （15JCZDJC33800）
Open Research Funding of Tianjin Key Lab. of Aqua-Ecology and Aquaculture (TJAE2015005) （TJAE2015005）
National Key Technology R&D Program (2011BAD13B04) （2011BAD13B04）
