四川医学2017，Vol.38Issue(8)：872-875,4.DOI:10.16252/j.cnki.issn1004-0501-2017.08.005
白藜芦醇对人视网膜色素上皮细胞氧化损伤的保护作用及机制研究
Study on Protective Effect and Mechanism of Resveratrol on Human Retinal Pigment Epithelial Cells Injured by Hy-drogen Peroxide
摘要
Abstract
Objective To observe the protective effect of resveratrol on injury induced by hydrogen peroxide oxidation of human retinal pigment epithelial cells.Methods The cultured human RPE cell line was divided into normal control group,hydro-gen peroxide damage group and different concentration resveratrol protective group.The normal control group without any treatment, hydrogen peroxide injury group with 200mg/L hydrogen peroxide,while the resveratrol protection group was added different concen-trations(12.5mg/L、25mg/L、50mg/L、100mg/L) and then was added 200μmol/L hydrogen peroxide.The cell inhibition rate was detected by CCK-8,and the contents of SOD and MDA in the cell culture medium were detected by SOD and MDA kits.Results The detectionby CCK-8 method showed that 50、100 mg/L of resveratrol for 2h,8h and 24h could significantly reduce the rate of cell inhibition,and compared with the oxidized injury group,the difference was statistically significant(P<0.05).The activity of 50mg/L、100mg/L of SOD in resveratrol protection groupwas significantly higher than that in the oxidative injury group,and the difference was statistically significant(P<0.05).The contents of 12.5mg/L、25mg/L、50mg/L、100mg/L of MDA at 2h,8h and 24h in resveratrol protective group were significantly lower than those in oxidized injury group,and the difference was statistically significant(P<0.01).Conclusion Resveratrol has protective effect on oxidative damage of RPE cells induced by hydrogen per-oxide,suggesting a role in the prevention and treatment of resveratrol on AMD.关键词
白藜芦醇/视网膜色素上皮细胞/过氧化氢/氧化损伤Key words
resveratrol/retinal pigment epithelial cells/hydrogen peroxide/oxidative damage分类
医药卫生引用本文复制引用
杨影,吴峥峥,曲超..白藜芦醇对人视网膜色素上皮细胞氧化损伤的保护作用及机制研究[J].四川医学,2017,38(8):872-875,4.基金项目
四川省科技厅应用基础研究项目(编号:30504010256) （编号:30504010256）
