山东医药2017，Vol.57Issue(41)：1-4,4.DOI:10.3969/j.issn.1002-266X.2017.41.001
乙型肝炎病毒X蛋白A1762T/G1764A双突变对肝癌细胞生物钟基因表达的影响
Effects of hepatitis B virus X protein A1762T / G1764A double mutation on expression of clock gene in liver cancer cells
摘要
Abstract
Objective To investigate the effects of hepatitis B virus X protein A1762T/ G1764A double mutation on the expression of clock gene in the hepatocellular carcinoma (HCC)cells. Methods Bel-7404 cells were inoculated into into 6-hole plate,when the cells grew to 60% confluence,they were transfected with 1. 0 μg pcDNA3. 1-HBx and pcD-NA3. 1-mHBx (A1762T/ G1764A double mutant)plasmids by using Lipofectamine 2000 according to the manufacturer's in-structions. The mRNA and protein expression levels of the CLOCK,BMAL1,Per1,Per2,Per3,Cry1,Cry2,and CKIεgenes in the Bel-7404 cells were detected by real-time PCR and Western blotting. Results The mRNA and protein expres-sion of Clock,Bmal1,Per2,Per3,Cry1 and Cry2 decreased in Bel-7404 cells transfected with A1762T/ G1764A double mutant HBx vectors as compared with that of cells transfected with pcDNA3. 1-HBx vectors,and the differences were statis-tically significant (all P < 0. 05). Conclusions HBx A1762T/ G1764A double mutation can cause the disorder of biologi-cal clock expression in HCC cells.关键词
肝肿瘤/乙型肝炎病毒X蛋白/突变/生物钟/钟控基因Key words
liver neoplasms/hepatitis B virus X protein/mutation/circadian clock/clock controlled gene分类
医药卫生引用本文复制引用
彭桦,占静,张乙凡,孙飞,邱梦君,杨盛力..乙型肝炎病毒X蛋白A1762T/G1764A双突变对肝癌细胞生物钟基因表达的影响[J].山东医药,2017,57(41):1-4,4.基金项目
国家自然科学基金资助项目(81702885) （81702885）
中央高校基本科研业务费专项资金项目(0118530328):华中科技大学自主创新基金项(2016YXM241) （0118530328）
中国肝炎防治基金会-天晴肝病研究基金科研课题(TQGB20170005) （TQGB20170005）
中国博士后科学基金资助项目(2017M613001). （2017M613001）
