山东医药2017，Vol.57Issue(41)：5-8,4.DOI:10.3969/j.issn.1002-266X.2017.41.002
融合蛋白SMP30-IP10真核表达质粒的构建及其对肝癌细胞迁移、侵袭、增殖的影响
Construction of eukaryotic expression plasmid containing fusion protein SMP30-IP10 and its effect on migration,invasion,and proliferation of liver cancer cells
摘要
Abstract
Objective To construct the eukaryotic expression plasmid of fusion protein SMP30-IP10,and to investi-gate its effects on migration,invasion,and proliferation of hepatoma cell line SK-hep1. Methods The SK-hep1 cells were divided into two groups:the experimental group which was transfected with pIRES-SMP30-IP10 plasmid,and the con-trol group which was transfected with empty pIRES. The eukaryotic expression plasmid pIRES-SMP30-IP10 was constructed by overlapping PCR,and then we transfected the plasimd into hepatoma cell line SK-hep1 by liposome transfection;the protein expression of cells was detected by Western blotting;the migration and invasion of cells were detected by Transwell assay;the proliferation of cells was detected by CCK8. Results The eukaryotic expression plasmid pIRES-SMP30-IP10 was successfully constructed and approved by PCR,double restriction enzyme digestion and DNA sequencing,and was ex-pressed in hepatocellular carcinoma cell line SK-hep1. Compared with the control group,the migration and invasion were inhibited to some extent in the experimental group (both P < 0. 05),but the morphology and proliferation had no significant change (both P > 0. 05). Conclusion The fusion protein SMP30-IP10 eukaryotic expression plasmid is successfully con-structed,and it can inhibit the migration and invasion abilitiesof hepatoma cells,but has no effect on the cell proliferation.关键词
肝肿瘤/融合蛋白/衰老标志蛋白30/干扰素诱导蛋白10/细胞迁移/细胞侵袭/细胞增殖Key words
liver neoplasms/fusion protein/senescence marker protein-30/interferon-inducible protein 10/cell mi-gration/cell invasion/cell proliferation分类
医药卫生引用本文复制引用
谢美玉,郭顺利,宾晓芸,石明霞,陈安宁,陈思宇,周素芳..融合蛋白SMP30-IP10真核表达质粒的构建及其对肝癌细胞迁移、侵袭、增殖的影响[J].山东医药,2017,57(41):5-8,4.基金项目
国家自然科学基金项目(81460432,81572994) （81460432,81572994）
广西自然科学基金项目(2015GXNSFDA139017) （2015GXNSFDA139017）
广西科学研究与技术开发计划项目(15104001-7) （15104001-7）
广西南宁青秀区科学研究与技术开发计划项目(2014S03). （2014S03）
