山东医药2017，Vol.57Issue(41)：16-19,4.DOI:10.3969/j.issn.1002-266X.2017.41.005
荷载CSCs抗原的DC疫苗联合小剂量TP治疗乳腺癌小鼠效果观察
Efficacy of CSCs antigens-loaded dendritic cell vaccine combined with low-dose TP in treatment of breast cancer in mice
摘要
Abstract
Objective To study the therapeutic effect and mechanism of cancer stem cells (CSCs)antigens-loaded dendritic cell (DC)vaccine combined with low-does TP (paclitaxel and cisplatin)in treatment of breast cancer in mice. Methods We sorted out the CSCs from breast cancer cell lines (4T1)by using ALDEFLUOR. Bone marrow-derived DCs were pulsed with CSCs lysate to generate CSCs-DC vaccine. The Balb/ c mice were inoculated with the heterogeneous 4T1 tumor cells by subcutaneous injection to make the mouse breast cancer models. The models were then divided into four groups:PBS group which was administrated PBS,TP group which was injected with 20 mg/ kg paclitaxel and 5 mg/ kg cis-platin intraperitoneally,CSCs-DC vaccine group (CSCs-DC group)which was administrated CSCs-DC vaccine by subcuta-neous injection,and the CSCs-DC vaccine + TP group (CSCs-DC + TP group)which was administrated CSCs-DC vaccine by subcutaneous injection and was injected with 20 mg/ kg paclitaxel and 5 mg/ kg cisplatin intraperitoneally. Tumor vol-umes were measured and survival was monitored. At the end of the experiment,the spleens in mice were harvested,the cy-totoxic lymphocyte (CTL)activity was measured by using LDH cytotoxicity assay,and IgG levels secreted by B cells was measured by ELISA. Results The tumor size in the CSCs-DC + TP group was less than that of the PBS group,the TP group and the CSCs-DC group (P < 0. 05). The average survival time of the CSCs-DC + TP group was (62. 00 ± 2. 65)d and was the longest,the average survival time of CSCs-DC group was (52. 00 ± 1. 00)d,the average survival time of TP group and PBS group were (46. 00 ± 2. 00)d and (35. 00 ± 1. 00)d,respectively. The CTL activity and IgG levels se-creted by B cells in the CSCs-DC + TP group were the highest,the CSCs-DC group was the second,TP group was lower than the CSCs-DC group and the CSCs-DC + TP group,but was higher than the PBS group,and the difference was statisti-cally significant (P < 0. 01). Conclusion The therapeutic effect of CSCs-DC vaccine combined with low-dose TP in the treatment of the mouse breast cancer is better than CSCs-DC vaccine and TP alone.关键词
乳腺肿瘤/肿瘤干细胞/疫苗/紫杉醇/顺铂/小鼠Key words
breast neoplasms/cancer stem cells/vccine/paclitaxel/cisplatin分类
医药卫生引用本文复制引用
陈心,梁慧玲,伍龙,徐细明,张一桥,陈能,冯翎..荷载CSCs抗原的DC疫苗联合小剂量TP治疗乳腺癌小鼠效果观察[J].山东医药,2017,57(41):16-19,4.基金项目
国家自然科学基金资助项目(81302133) （81302133）
中央高校基本科研业务费专项基金资助项目(2042014kf0149) （2042014kf0149）
湖北省自然科学基金资助项目(2012FKC143). （2012FKC143）
