山东医药2017，Vol.57Issue(42)：9-12,4.DOI:10.3969/j.issn.1002-266X.2017.42.003
全反式维甲酸在成年小鼠神经干细胞分化中的调控作用及机制
Regulation of all-trans-retinoic acid on differentiation of neural stem cells in adult mice
摘要
Abstract
Objective To investigate the effects of all-trans-retinoic acid (ATRA)on signaling pathway and regula-tions of neural stem cells (NSCs)in adult mice and the effects on the family of cytochrome P450 (CYP450). Methods NSCs were isolated from the sub-ventricular zone (SVZ)of brain in adult mice and then were cultured. The cells were di-vided into the ATRA group and blank control group. The ATRA group was added with 10 - 6mol/L ATRA. Flow cytometry (FCM)was used to detect the cell surface markers in the two groups,and we calculated the percentage of neurons,astro-cytes,oligodendrocytes and the percentage of NSCs accounting for total cells. The real-time PCR was used to detect the re-lated gene expression of CYP450 family,and we screened statistically significant gene. ELISA was applied to detect the ac-tivity of cytochrome P450 reductase (CPR). The expression levels of P38 and p-P38 were detected by using Western blot-ting. Results In the ATRA group,NSCs mainly differentiated into neurons,and some differentiated into astrocytes,with less differentiation of oligodendrocytes. Compared with the blank control group,the expression of CYP26A1,CYP26B1 and CYP26C1 in the P450 family genes of the ATRA group was up-regulated (all P < 0.05). The activity of CPR and the ex-pression of p-P38 protein in the ATRA group were higher than those in the blank control group (both P <0.05). Conclu-sion ATRA promotes the differentiation of mouse NSCs into neurons,and it may play a regulatory role by up-regulating the CYP26 family genes in CYP450 and increasing CPR activity and the P38 MAPK pathway.关键词
全反式维甲酸/神经退行性疾病/神经干细胞/细胞色素P450/细胞色素P450还原酶/P38MAPK通路Key words
all-trans-retinoic acid/neurodegeneration/neural stem cells/cytochrome P450/cytochrome P450 reduc-tase/P38 MAPK pathway分类
医药卫生引用本文复制引用
李先红,胡鹤娟,唐曦瀛,吴坚,黄金忠,姚允怡..全反式维甲酸在成年小鼠神经干细胞分化中的调控作用及机制[J].山东医药,2017,57(42):9-12,4.基金项目
江苏省自然科学基金资助项目(BK20130219) （BK20130219）
江苏高校"青蓝工程"科研资助项目. （）
