山东医药2017，Vol.57Issue(42)：16-19,4.DOI:10.3969/j.issn.1002-266X.2017.42.005
薯蓣皂苷对哮喘小鼠气道上皮细胞糖皮质激素受体表达的影响
Effect of dioscin on glucocorticoid receptor of airway epithelial cells in mice with asthma
摘要
Abstract
Objective To observe the effects of dioscin on the glucocorticoid receptor GRα and GRβ of airway epithe-lial cells in mouse models with asthma,and to investigate its anti-inflammatory effect on asthma. Methods Normal mouse airway epithelial cells were used as the blank control group,and the airway epithelial cells from asthmatic mouse models were divided into 3 groups as following:asthmatic non-treatment group (cells cultured in a medium containing no drug), asthmatic treatment group (cells cultured in a medium containing dioscin),and asthmatic antagonistic group (cells cul-tured in medium containing dioscin and GR antagonists,RU486). The levels of IL-1,IL-6 and TNF-α in the conditional media were measured by using ELISA. The mRNA and protein levels of GRα,GRβ,and HSP90 in the cells were detected by using real-time quantitative PCR and Western blotting,respectively. Results The levels of IL-1,IL-6,and TNF-αwere significantly higher in the asthmatic non-treatment group than in the asthmatic treatment group and the blank control group,and the levels of IL-1,IL-6,and TNF-α were higher in the asthmatic antagonistic group than in the asthmatic treat-ment group (all P <0.05). The expression of GRα,GRβ,and HSP90 at protein and mRNA levels was significantly lower in the asthmatic treatment group than in the blank control group (all P<0.05). The expression of GRα was higher,while the expression of GRβ and HSP90 at protein and mRNA levels was lower in the asthmatic treatment group than in the asth-matic non-treatment group (all P < 0.05). The expression of GRα,GRβ,and HSP90 at protein and mRNA levels of the asthmatic antagonistic group had no statistic difference as compared with that of the asthmatic non-treatment group. Conclu-sion Dioscin exerts its anti-inflammatory effects in asthmatic mice by regulating the expression of GRα,GRβ,and HSP90 in airway epithelial cells.关键词
哮喘/薯蓣皂苷/气道上皮细胞/糖皮质激素受体/热休克蛋白90/小鼠Key words
asthma/dioscin/airway epithelial cells/glucocorticoid receptor/heat shock protein 90/mice分类
医药卫生引用本文复制引用
李先茜,吴宁,余荣环,管超,徐婷,吴嘉熙..薯蓣皂苷对哮喘小鼠气道上皮细胞糖皮质激素受体表达的影响[J].山东医药,2017,57(42):16-19,4.基金项目
上海市卫生和计划生育委员会科研项目(20134206). （20134206）
