山东医药2017，Vol.57Issue(43)：14-16,3.DOI:10.3969/j.issn.1002-266X.2017.43.005
冬凌草甲素对人脑胶质瘤细胞株U251杀伤、增殖及NF-κB蛋白表达的影响
Effects of oridonin on killing,proliferation,and expression of NF-κB protein of human glioma U251 cell line
摘要
Abstract
Objective To observe effects of oridonin on killing,proliferation,and expression of NF-κB protein of hu-man glioma U251 cell line. Methods U251 cells were incubated with oridonin at the concentrations of 10,20,40,and 80 μmol/ L respectively. At the same time,U251 cells incubated without oridonin were taken as the control group. U251 counting,IC 50 ,U251 cell proliferation inhibition rate and expression of NF-κB were detected at different time points. Re-sults Compared with the control group,the number of U251 cells in the 40 and 80 μmol/ L oridonin groups decreased sig-nificantly (P < 0. 05). After incubation for 24,48 and 72 h,the IC 50 of U251 cells was 82. 2,65. 3 and 44. 1 μmol/ L, respectively. Compared with 20 μmol/ L oridonin group,the cell proliferation inhibition rates of the 40 and 80 μmol/ L ori-donin groups were significantly reduced at 48 and 72 h (P < 0. 05). Compared with the control group,the expression of NF-κB in U251 cells decreased significantly (P < 0. 05)in the 40 and 80 μmol/ L oridonin group at 48 and 72 h. Conclu-sions Oridonin can kill U251 cells,inhibit its proliferation and down-regulate NF-κB protein in vitro. The killing effect and inhibition can be related to the regulation of NF-κB signaling pathway.关键词
冬凌草甲素/脑胶质瘤/细胞生长/细胞增殖/NF-κB信号通路/NF-κB蛋白Key words
oridonin/glioma/cell growth/cell proliferation/NF-κB signaling pathway/NF-κB protein分类
医药卫生引用本文复制引用
苑鹤毅,王丽敏,刘君,林大勇,刘鸿,白剑,张丽艳..冬凌草甲素对人脑胶质瘤细胞株U251杀伤、增殖及NF-κB蛋白表达的影响[J].山东医药,2017,57(43):14-16,3.基金项目
辽宁省自然科学基金资助项目(201602506). （201602506）
