世界中医药2017，Vol.12Issue(9)：2151-2154,2159,5.DOI:10.3969/j.issn.1673-7202.2017.09.040
电针对慢性束缚应激抑郁模型大鼠不同时段血清ACTH、CORT表达的影响
Effects of Electro-acupuncture on the Expression of Serum ACTH and CORT at Different Time in Rats Exposed to Chronic Restraint Stress
摘要
Abstract
Objective:We observed the effects of electro-acupuncture on the expression of serum ACTH and CORT in rats exposed to chronic restraint stress (CRS),to explore the antidepressive effects and possible mechanisms of electro-acupuncture in the treatment of depression.Methods:Sixty SD rats were equally divided into three groups:control group,model group and electro-acupuncture group.Except the control group,all rats were in the condition of chronic restraint stress and separation.The EA group was stimulated at GV 20 and EX-HN 3 for 20 min before daily bound.ACTH and CORT levels were detected by ELISA respectively on the 7th,28th,35th day (the 7th day after the last intervention).Results:Effects on Serum ACTH and CORT:After 7 days,there was no difference among three groups.On the 28th and the 35th day,compared with the control group,the model group significantly increased (P ＜0.01;P ＜ 0.01;P ＜0.01;P ＜ 0.01);Compared with the model group,EA group significantly decreased (P ＜0.05;P ＜0.05;P ＜0.01;P ＜0.01).The electro-acupuncture had a reversal effect on the expression of serum ACTH and CORT in rats exposed to chronic restraint stress.Conclusion:The hypothalamic-pituitary-adrenal (HPA) axis hyperactivity is observed in rats exposed to chronic restraint stress,and its level of serum ACTH and CORT are significantly higher.The level of serum ACTH and CORT are reversed by EA intervention,which suggests that EA intervention can regulate HPA axis hyperactivity.The 7th day after the last intervention,the level of ACTH and CORT of EA group are still significantly lower than the model group,which suggests that EA intervention has continuous regulatory effect on the HPA axis hyperactivity,and it may be one of the persistent effect mechanisms of antidepressive effects of EA.关键词
抑郁症/电针/慢性束缚应激/促肾上腺皮质激素/肾上腺皮质酮Key words
Depression/Electro-acupuncture/Chronic restraint stress/ACTH/CORT分类
医药卫生引用本文复制引用
梁星辰,冯世醒,卢峻,图娅,孙岚,王瑜,姜会梨,李晶,杨昕婧,赵冰骢,俞秋云,李想..电针对慢性束缚应激抑郁模型大鼠不同时段血清ACTH、CORT表达的影响[J].世界中医药,2017,12(9):2151-2154,2159,5.基金项目
国家自然科学基金项目(81173334,81373729) （81173334,81373729）
北京市优秀博士学位论文指导教师科研项目(YB20091002601) （YB20091002601）
