森林工程2017，Vol.33Issue(6)：11-14,4.
黄檗雌雄植株增殖培养性别差异研究
Study on the Difference of the Rapid Propagation of Phellodendron amurense Rupr . in Dioecious
摘要
Abstract
In order to solve the Phellodendron amurense reproduction barrier and to protect germplasm resources and solve the problem of shortage of nursery stock , we chose Phellodendron amurense new initiation branch stem segments as explants to discuss the different sterilization methods, sampling time, the influence of gender on cultivating Phellodendron amurense propagation.The results showed that the best medium for the cultivation of Phellodendron amurense was MS+1.0mg/L6-BA+0.2mg/L NAA+1.0mg/L GA3 and the induction rate was more than 80%.Sterilized the explants of Phellodendron amurense in 0.1%HgCl2 for 8 min could reach a good effect and no harm to seedling growth .The best sampling time is May .The propagation culture of the male and female plants was remarkable , and the average breeding rate of the female was about two to three times than the male .Phellodendron amurense reproduc-tion technology was incomplete , and the tissue culture technology is the best way to solve the shortage of germplasm resources .Phello-dendron amurense plant propagation technology has been successful with new initiation branches , and needs further study on the Phello-dendron amurense tube seedlings rooting problems .关键词
黄檗/雌雄植株/增殖培养/差异Key words
Phellodendron amurense/male and female plant/rapid propagation/difference分类
农业科技引用本文复制引用
张玲,张东来,邓晓华..黄檗雌雄植株增殖培养性别差异研究[J].森林工程,2017,33(6):11-14,4.基金项目
国家自然科学基金资助项目(31600485) （31600485）
黑龙江省省属科研院所基本科研业务费专项(2016-06) （2016-06）
