生命科学研究2017，Vol.21Issue(5)：377-381,5.DOI:10.16605/j.cnki.1007-7847.2017.05.001
濒危物种绒毛皂荚组织培养快繁技术的初步研究
A Preliminary Study on Tissue Cultures of Endangered Species Gleditsia vestita Chun et How ex B.G.Li
摘要
Abstract
The seeds of Gleditsia vestita Chun et How ex B.G.Li were used as explant material.Through callus differentiation,adventitious bud multiplication,acclimatization and transplantation,the tissue culture system was set up.The results are as follows:1) The best group appeared in the medium containing MS+6-BA 3.0 mg/L+KT 0.4 mg/L+IAA 0.3 mg/L,in which the callus and differentiated adventitious buds were induced synchronously from the cotyledons and hypocotyls.The callus induction rate reached 90.5％ and synchronous differentiation rate reached 77.9％;2) The average proliferation times of buds were 4.0 in the medium containing 1/2MS+6-BA 1.0 mg/L+IBA 0.3 mg/L+NAA 0.2 mg/L;3) The rooting rate was 100％ in MS+NAA 0.5 mg/L+IAA 0.25 mg/L.The plantlets grew well with 90％ survival after transplantation.关键词
濒危物种/绒毛皂荚/种子/组织培养/正交设计Key words
endangered species/Gleditsia vestita Chun et How ex B.G.Li/seed/tissue culture/orthogonal design分类
生物科学引用本文复制引用
田学辉,黄丽芳,周柳,吴尧晶,刘克明..濒危物种绒毛皂荚组织培养快繁技术的初步研究[J].生命科学研究,2017,21(5):377-381,5.基金项目
国家自然科学基金资助项目(30470130) （30470130）
国家科技基础性研究专项(2013FY111500) （2013FY111500）
湖南省林木种质资源调查(湘财农指(2015)91号) （湘财农指(2015）
湖南省外来入侵生物调查(湘财农指(2014)91号) （湘财农指(2014）
湖南省农业野生植物资源调查(湖南省农业厅) （湖南省农业厅）
湖南省生态学重点学科建设项目(0713) （0713）
