生命科学研究2017，Vol.21Issue(5)：382-385,4.DOI:10.16605/j.cnki.1007-7847.2017.05.002
以Aβ4-11为免疫原制备Aβ1-42多克隆抗体
The Preparation of Polyclonal Antibody Against Aβ1-42 with Aβ4-11 as Immunogen
摘要
Abstract
β amyloid 1-42 (Aβ1-42) is a causative protein of Alzheimer's disease (AD).Its antibody research and development have been under study.Herein,two-month old female rabbits were immunized with Aβ4-11 coupled with KLH.The anti-Aβ4-11 IgG titer in the sera of female rabbits was detected by ELISA.Protein G-Sepharose affinity chromatography was used to purify the IgG antibodies.Bradford method and SDS-PAGE were used to determine the content and the purity of IgG,respectively.Finally,the polyclonal antibodies were used to detect the senile plaques in normal mice and nine-month old APP transgenic mice by immunohistochemistry to determine the specificity and affinity of the antibody against Aβ1-42.The results showed that Aβ4-11 had good humoral immunogenicity and good specificity and affinity for Aβ1-42.The results suggest that the Aβ1-42 polyclonal antibody prepared with Aβ4-11 subunit fragment as immunogen can be used as an effective tool for studying AD,and also provides a new possibility in diagnosis and treatment of AD.关键词
阿尔茨海默病(AD)/β淀粉样蛋白1-42 (Aβ1-42)/β淀粉样蛋白4-11 (Aβ4-11)/免疫原/抗体制备Key words
Alzheimer's disease (AD)/β amyloid 1-42 (Aβ1-42)/β amyloid 4-11 (Aβ4-11)/immunogen/antibody preparation分类
生物科学引用本文复制引用
张松江,赵献敏,王权,吕明惠,苏少华..以Aβ4-11为免疫原制备Aβ1-42多克隆抗体[J].生命科学研究,2017,21(5):382-385,4.基金项目
河南省科技厅重点科技攻关项目(152102310100) （152102310100）
