生命科学研究2017，Vol.21Issue(5)：386-391,6.DOI:10.16605/j.cnki.1007-7847.2017.05.003
全细胞膜片钳研究离体小鼠内侧膝状体亚核神经元电生理特性
In Vitro Whole-cell Patch Clamp Recordings of Neurons in Subnuclei of Mouse Medial Geniculate Body
摘要
Abstract
Medial geniculate body (MGB) is an important thalamic relay along the auditory pathway.To investigate the electrophysiological properties of MGB neurons,99 MGB neurons were obtained by using in vitro whole-cell patch clamp recording technique,including 36 neurons from the ventral division of the MGB (MGBv),34 neurons from the dorsal division of the MGB (MGBd) and 29 neurons from the medial division of the MGB (MGBm).Results showed that two firing patterns including bursting (16.2％,16/99) and tonic (83.8％,83/99) were identified based on the responses to depolarizing current,and that depolarizing rebounds were found at the offset of hyperpolarizing currents in all of the MGB neurons.Only some of the neurons (17.2％,17/99) also showed hyperpolarization-activated inward current (Ih) to hyperpolarizing currents.Moreover,the resting membrane potential (RMP) and active threshold showed significant differences among neurons of different subnuclei (P＜0.05).These results indicate that MGB subnuclear neurons are different in electrophysiological properties.This would help us understand the distinct functions of MGB neurons in central auditory processing.关键词
小鼠/全细胞膜片钳/内侧膝状体(MGB)/发放模式/静息膜电位(RMP)/激活阈值Key words
mouse/whole-cell patch clamp/medial geniculate body (MGB)/firing pattern/resting membrane potential (RMP)/active threshold分类
生物科学引用本文复制引用
杜小凤,贾慧娟,王欣..全细胞膜片钳研究离体小鼠内侧膝状体亚核神经元电生理特性[J].生命科学研究,2017,21(5):386-391,6.基金项目
华中师范大学基本科研业务费青年教师项目(CCNU15A05035) （CCNU15A05035）
