生命科学研究2017，Vol.21Issue(5)：399-404,6.DOI:10.16605/j.cnki.1007-7847.2017.05.005
拟南芥LecRK-ConA基因突变体的筛选鉴定及其功能初步研究
The Mutant Identification and Preliminary Functional Study of LecRK-ConA Gene in Arabidopsis thaliana
摘要
Abstract
Lectin receptor-like kinases (LecRLKs) play an important role in plant resistance,hormone signal pathways and plant growth.The LecRK-ConA gene is a member of the Arabidopsis LecRLK family.Here,three primer PCR methods were used to identify the homozygous T-DNA mutants of LecRK-ConA gene.The analysis of cis-elements in promoter showed that LecRK-ConA gene had multiple drought,light and GA responsive elements.The results of real-time quantitative PCR showed that the expression of LecRK-ConA gene had tissue differences and it had the strongest expression in seeds after vernalization for four days.The expression of LecRK-ConA gene was inhibited under NaCl and mannitol treatments in A rabidopsis.The seed germination rate of lecrk-cona was lower than that of the wild type under NaCl and mannitol treatments.Furthermore,lecrk-cona mutants flowered early compared to Col-0 plants.The results provide a basis for further study on the function of LecRK-ConA gene involved in stress tolerance and flowering regulation.关键词
拟南芥/LecRK-ConA基因/凝集素类受体激酶(LecRLKs)/逆境胁迫/开花调节Key words
A rabidopsis/LecRK-ConA gene/lectin receptor-like kinases (LecRLKs)/stress/flowering regulation分类
生物科学引用本文复制引用
何壮,李金燕,张骋,肖文君,刘书灿,李美玲,郭新红..拟南芥LecRK-ConA基因突变体的筛选鉴定及其功能初步研究[J].生命科学研究,2017,21(5):399-404,6.基金项目
国家自然科学基金资助项目(31540064,31071076,30871325) （31540064,31071076,30871325）
教育部高等学校博士学科点专项科研基金(20130161110005) （20130161110005）
植物分子遗传国家重点实验室资助项目(2015) （2015）
湖南省研究生科研创新项目(CX2017B145,CX2015B073,CX2016B097) （CX2017B145,CX2015B073,CX2016B097）
湖南省重点研发计划项目(2016WK2003) （2016WK2003）
国家重点研发计划项目子课题(2017YFF0210301) （2017YFF0210301）
