生命科学研究2017，Vol.21Issue(5)：405-411,7.DOI:10.16605/j.cnki.1007-7847.2017.05.006
山核桃叶总黄酮抑制TBBPA诱导斑马鱼胚胎发育毒性的研究
Protective Effects of Total Flavonoid Against Tetrabromobisphenol A-induced Developmental Toxicity in Zebrafish Embryo-Larvae
摘要
Abstract
Tetrabromobisphenol A (TBBPA) is one of the highly concerned emerging contaminants.It has fierce toxicity to fish.Leaves of Carya cathayensis Sarg.have showed obvious antioxidant and anti-apoptosis effects as they contain large quantity of flavonoid compounds.Herein,total flavonoid compounds were separated and extracted from leaves of Carya cathayensis Sarg.through silica gel column elution and thin layer chromatography (TLC) detection.Four experimental groups (blank control,0.5 mg/L of TBBPA,1.0 mg/L of total flavonoid compounds,0.5 mg/L of TBBPA and 1.0 mg/L of total flavonoid compounds) were set up.During the development of zebrafish embryo under TBBPA exposure,indices of morphological toxicity were recorded,reactive oxygen species (ROS) production level and apoptotic gene expression were analyzed.Results indicated that 22 types of total flavonoid compounds were found,mainly including 50％ chalcone,27％flavonol,18％ flavanone and 5％ isoflavone.Morphological toxicity indices induced by TBBPA,such as spawn coagulation rate,malformation rate and blood flow disorders,were improved significantly after treatment with total flavonoid compounds.Meanwhile,the rising ROS level induced by TBBPA was significantly inhibited,and the up-regulated expression of p53 and down-regulated expression of Bcl-2 induced by TBBPA were also inhibited.Total flavonoid in leaves of Carya cathayensis Sarg.were found to have antioxidant and anti-apoptosis effects by decreasing the level of ROS and inhibiting abnormal expressions of apoptosis genes.The results contributed to the acquaintance of protective effects of total flavonoid,which are the active ingredients in leaves of Carya cathayensis Sarg.,against TBBPA-induced developmental toxicity in zebrafish embryo and larvae,providing a new idea for protecting aquatic ecosystems and human health.关键词
山核桃叶/总黄酮/四溴双酚A (TBBPA)/斑马鱼/胚胎发育/毒性分析Key words
Carya cathayensis Sarg.leaf/total flavonoid/tetrabromobisphenol A (TBBPA)/zebrafish/embryonic development/toxicity analysis分类
生物科学引用本文复制引用
金位栋,杨苏文,孙丰超,丁志山,闫玉红,杜家慧..山核桃叶总黄酮抑制TBBPA诱导斑马鱼胚胎发育毒性的研究[J].生命科学研究,2017,21(5):405-411,7.基金项目
国家水专项“十二五”湖泊主题滇池项目(2012ZX07102-004) （2012ZX07102-004）
