生命科学研究2017，Vol.21Issue(5)：433-436,4.DOI:10.16605/j.cnki.1007-7847.2017.05.011
小干扰RNA抑制APP的表达对鼻咽癌CNE2细胞增殖和迁移的影响
Effects of Silencing APP Gene Expression by RNA Interference on Proliferation and Migration of Nasopharyngeal Carcinoma CNE2 Cells
摘要
Abstract
To investigate the roles of amyloid precursor protein (APP) in nasopharyngeal carcinoma CNE2 cells,siRNA expressing plasmids were transfected into CNE2 cells by Lipofectamine 2000 reagent,and a nonspecific plasmid was used as control.Immunofluorescence and Western-blot were used to detect the transfection efficiency and the expression level of APP protein,respectively.MTT test and wound healing assay were performed to evaluate the proliferation and migration abilities of CNE2 cells.The results showed that APP protein was significantly lower in the interference group than that in the control group (P＜0.05).Interference with APP gene could inhibit the proliferation and reduce the healing of scratches of CNE2 cells significantly.These results suggested that RNAi could effectively inhibit the expression of APP protein,and that proliferation and migration of CNE2 cells could be suppressed by inhibiting APP expression.The study provides a new clue for gene therapy on nasopharyngeal carcinoma.关键词
RNA干扰技术(RNAi)/鼻咽癌(NPC)/淀粉样蛋白前体(APP)/细胞增殖/细胞迁移Key words
RNA interference (RNAi)/nasopharyngeal carcinoma (NPC)/amyloid precursor protein (APP)/cell proliferation/cell migration分类
医药卫生引用本文复制引用
赵师,王永强,刘芬,汤参娥..小干扰RNA抑制APP的表达对鼻咽癌CNE2细胞增殖和迁移的影响[J].生命科学研究,2017,21(5):433-436,4.基金项目
国家自然科学基金(81570776) （81570776）
湖南省科技计划一般项目(2013FJ4112) （2013FJ4112）
