生命科学研究2017，Vol.21Issue(5)：437-441,449,6.DOI:10.16605/j.cnki.1007-7847.2017.05.012
丹参酸甲对小鼠骨髓来源树突状细胞表型及其部分功能的影响
Effect of Salvianic Acid on the Phenotype and Partial Immune Functions of Mouse Bone Marrow-derived Dendritic Cells
马效雷 1于晓锋 2牛文堂 3马文华 2马文翠 2胡涛4
作者信息
- 1. 滨州医学院免疫学教研室,中国山东烟台264003
- 2. 菏泽市立医院心内一科,中国山东菏泽274000
- 3. 淄博市临淄区人民医院临床微生物科,中国山东淄博255000
- 4. 菏泽市第三人民医院内二科,中国山东菏泽274000
- 折叠
摘要
Abstract
To explore the effect of salvianic acid on the cell phenotype and partial immune functions of mouse bone marrow-derived dendritic cells (DCs),mouse bone marrow cells were separated and culutred in vitro firstly,and were then induced to differentiate into DCs by IL-4 and GM-CSF.The percentage,MHC-Ⅱ,CD80 and CD86 expression levels and phagocytosis of these DCs were detected by flow cytometry before and after treatment with salvianic acid A.The cytokine concentrations in the culture supernatant of DCs co-cultured with T cells were examined by ELISA.Results showed that the percentage of DCs and the number of FITC-Dextran positive cells were obviously increased,while the ratio of MHC-Ⅱhigh/MHC-Ⅱlow,the mean flourescence intensity of MHC-Ⅱhigh and MHC-Ⅱlow and the expression levels of CD80 and CD86 on DCs were decreased significantly after treatment with salvianic acid A.In addition,salvianic acid A enhanced the secretion ability of IL-10,and inhibited INF-γ secretion ability by T cells stimulated by DCs.These results indicate that salvianic acid A could induce bone marrow cells to differentiate into DCs,but inhibit DC maturation and its antigen presenting funcion,resulting in decreased expression of surface marker on DCs.Besides,salvianic acid A could strengthen the ability of antigen uptake and phagocytosis of DCs,and alleviate the inflammatory reaction mediated by DC and T cell interactions.These results will provide strong support for the administration of salvianic acid A on the immune diseases induced by T cell excessive activation.关键词
小鼠/丹参酸甲/树突状细胞(DC)/细胞表型/炎症反应/炎性因子Key words
mouse/salvianic acid/dendritic cell (DC)/phenotype/inflammatory reaction/inflammatory factor分类
医药卫生引用本文复制引用
马效雷,于晓锋,牛文堂,马文华,马文翠,胡涛..丹参酸甲对小鼠骨髓来源树突状细胞表型及其部分功能的影响[J].生命科学研究,2017,21(5):437-441,449,6.
