水生态学杂志2017，Vol.38Issue(6)：48-56,9.DOI:10.15928/j.1674-3075.2017.06.007
纤细裸藻对5种抗生素的胁迫响应
Response of Euglena gracilis to Five Kinds of Antibiotic Stress
摘要
Abstract
As an excellent nutritional resource, the microalgae Euglena gracilis is rich in complex nutrients, inclu-ding amino acids, unsaturated fatty acids and vitamins.Additionally, the nutrients derived from E.gracilis are more easily absorbed by humans and aquatic animals than from other algae because it has no cell walls.Therefore, it is important to develop intensive cultivation methods and optimize the breeding environment for E.gracilis.In this study, we investigated the variation of cell density and chlorophyll-a ( Chl-a) levels of E.gracilis cultured ex-posed to different concentrations of five antibiotics: Geneticin, Chloramphenicol, Penicillin, Oxytetracycline and Streptomycin, to explore sterile culture methods and provide basic data for establishing a sterile system for E.graci-lis.The experiment was carried out under laboratory conditions.E.gracilis was cultured to the logarithmic phase for the test, with a cell density of 5 ×105 cells/mL under the following conditions:temperature of (25 ±1)℃, illu-mination of 60μmol/(m2· s) and a light-dark cycle of 12:12.Antibiotics were added to culture solutions to give Chloramphenicol, Penicillin, Oxytetracycline and Streptomycin concentrations of 25, 50, 75, 100, 200 μg/mL and geneticin concentrations of 25, 50, 75, 100, 125μg/mL and each treatment was run in triplicate.After treat-ment, culture solutions were sampled once a day for determination of cell density and Chl-a content.Geneticin, Oxytetracycline, and Streptomycin significantly decreased cell density ( P<0.05) and Geneticin, at concentrations under 25 μg/mL, significantly inhibited growth and Chl-a synthesis.Oxytetracycline (200 μg/mL) and Strepto-mycin (25-50μg/mL) increased Chl-a content, while Chloramphenicol and Penicillin promoted growth.Penicil-lin (200 μg/mL) significantly enhanced cell density and Chl-a levels (P<0.05).Results indicate that E.graci-lis is sensitive to Geneticin, Oxytetracycline, and Streptomycin and that Chloramphenicol and Penicillin can be used as antibiotics for axenic culture of E.gracilis.The results will also serve as reference for identifying a selec-tive marker of E.gracilis genetic engineering and the axenic culture of microalgae.关键词
抗生素/纤细裸藻/胁迫/响应Key words
antibiotic/Euglena gracilis/stress/response分类
生物科学引用本文复制引用
张文慧,窦勇,高金伟,姜智飞,贾旭颖,周文礼,王永苓,高建忠..纤细裸藻对5种抗生素的胁迫响应[J].水生态学杂志,2017,38(6):48-56,9.基金项目
天津市水产产业技术体系创新团队项目(ITTFRS2017005) （ITTFRS2017005）
天津市科技重大专项与工程项目(15ZXBFNC00120) （15ZXBFNC00120）
卫星海洋环境动力学国家重点实验室开放基金项目(SOED1419) （SOED1419）
农业部南海渔业资源开发利用重点实验室开放基金(FREU2015-04) （FREU2015-04）
农业部水产品加工重点实验室开放基金(NYGJ201508) （NYGJ201508）
天津农学院研究生创新培育项目(2017YPY031). （2017YPY031）
