实用肝脏病杂志2017，Vol.20Issue(6)：663-667,5.DOI:10.3969/j.issn.1672-5069.2017.06.007
小檗碱对酒精性肝炎小鼠"肠漏"的保护作用
Protective effect of berberine on intestinal leak in mice with alcoholic hepatitis
摘要
Abstract
Objective To explore the protective effect of berberine on intestinal leak in mice with alcoholic hepatitis. Methods Forty male C57BL/6 mice were randomly divided into control,alcohol,berberine and alcohol plus berberine group with 10 animals in each. Mice were fed with alcohol or vehicle for 8 weeks and all were sacrificed. Serum alanine aminotransferas(ALT),aspartate aminotransferase(AST)and lipopolysaccharide(LPS) were detected by enzyme-linked immunosorbent assay(ELISA). The pathologic changes of liver tissues were assessed by HE staining,and the ultrastructures of tight junction(TJ)complex was observed by transmission electron microscope. In vitro,occludin,the main structural protein of intercellular TJ,was detected by immunofluorescence in intestinal epithelial Caco-2 cells,which were cultured with ethanol(EtOH),BBR and EtOH plus BBR respectively. Results Serum ALT,AST and LPS levels in alcohol group were(46.5±5.9)U/L,(57.0± 6.1)U/L and(0.40±0.05)ng/ml,respectively,significantly higher than those in control group(P<0.01);There was no significant difference between alcohol plus BBR group and control group;Serum ALT,AST and LPS levels in alcohol plus BBR group were(27.6±4.2)U/L,(31.8±4.1)U/L and(0.24 ±0.03)ng/ml,respectively,significantly lower than those in alcohol group(P<0.05);The histopathologic examination of liver tissues showed alcoholic hepatitis in alcohol group,and it was alleviated as respect to steatosis and inflammatory cell infiltration in alcohol plus BBR group;Electron micrographs showed that the TJ structures between intestinal epithelial cells were fuzzy and the gaps were expanded obviously in alcohol group,and all of these were improved obviously in alcohol plus BBR group;Immunofluorescence indicated that the occludin on Caco-2 cell membranes were decreased,interrupted and partly entered into cytoplasm in alcohol group, while in alcohol plus BBR group,the distribution of occludin was decreased,though,but was continuous.Conclusion Berberine improves the decrease in occludin expression on cell membranes and the disruption of TJ structures between intestinal epithelial cells induced by chronic ethanol intake and alleviates intestinal leak.关键词
酒精性肝炎/小檗碱/肠漏/紧密连接/Occludin蛋白/小鼠Key words
Alcoholic hepatitis/Berberine/Intestinal leak/Tight junction/Occludin/Mice引用本文复制引用
王洪岩,迟程,徐有青,李鑫..小檗碱对酒精性肝炎小鼠"肠漏"的保护作用[J].实用肝脏病杂志,2017,20(6):663-667,5.基金项目
北京市自然科学基金资助项目(编号:7154194) （编号:7154194）
国家自然科学基金资助项目(编号:81570536) （编号:81570536）
