摘要
Abstract
Objective To investigate the JAG1 protein expression on the prognosis of patients with intrahepatic cholangiocellular carcinoma(CCC). Methods 60 patients with intrahepatic cholangiocarcinoma in our hospital between Jan 2010 and Dec 2014 were included and 60 patients with benign biliary diseases who were treated in our hospital during the same period were selected as control. JAG1 mRNA and its protein in liver tissues were detected. Serum JAG1 level was also measured by ELISA. Laboratory and clinical pathological parameters were recorded and the correlations of them to JAG1 were calculated. Univariate and multivariate unconditional Logistic regression analysis of risk factors for postoperative prognosis in patients with CCC was conducted. Results In paracancerous tissues,liver tissues and cholangiocarcinoma tissues,the positive expression rates of JAG1 protein were 45.0%(27/60),56.7%(34/60)and 81.7%(49/60),respectively,and the relative levels of JAG1 mRNA were(0.91±0.34)and(0.57±0.15),(1.15±0.48)and(0.84±0.47),(1.96 ±0.58)and(1.64±0.58), respectively,(P<0.05);serum JAG1 level in patients with CCC was(205.35±22.51)pg/ml,significantly higher than that in the controls [(102.56 ±16.52)pg/ml,P<0.05];serum JAG1 level in patients with intrahepatic CCC was positively correlated with CA125(r=0.41,P<0.05),CEA(r=0.0.27,P<0.05),CRP(r=0.17,P<0.05),tumor invasion depth(r=0.13,P<0.05),lymph node metastasis(r=0.08,P<0.05),TNM stage(r=0.14,P<0.05)and tumor differentiation (r=0.15,P<0.05);multivariate Cox proportional hazard regression model analysis showed that the depth of tumor invasion,lymph node metastasis,TNM staging, differentiation,high serum JAG1 level were the risk factors of relapse free survival(RFS)in patients with bile duct carcinoma(P<0.05);the depth of tumor invasion,lymph node metastasis,TNM staging,high serum JAG1 levels were the risk factors of overall survival (OS)in patients with bile duct carcinoma(P<0.05). Conclusion JAG1 protein can be used as a specific marker for the prognosis of patients with intrahepatic cholangiocarcinoma after surgical resection and patients with high serum JAG1 levels may have a poor prognosis.关键词
胆管细胞癌/JAG1蛋白/预后Key words
Cholangiocellular carcinome/JAG1 protein/Prognosis
