太原理工大学学报2017，Vol.48Issue(6)：929-933,945,6.DOI:10.16355/j.cnki.issn1007-9432tyut.2017.06.009
GFP遗传改造四膜虫细胞的构建及其对重金属污染的荧光响应
Construction of Genetically Modified Tetrahymena with GFP and Its Fluorescence in Response to Environmental Heavy Metal Pollution
摘要
Abstract
To obtain genetically modified cells which can be used to detect a variety of environ-mental heavy metals pollution,the fusion gene containing heavy metals induced metallothionein gene MTT1 promoter and exogenous gfp reporter gene was transfected into Tetrahymena ther-mophilia by biolistic bombardment.By homologous recombination under increasing paromomy-cin,the genetically modified Tetrahymena with GFP was constructed.With treatment of Cd2 + , the MTT1 promoter can initiate the correct expression of exogenous gfp gene of the genetically modified Tetrahymena in which green fluorescence can be induced by blue light excitation.The fluorescence response of the genetically modified Tetrahymena with GFP to heavy metals was in order of Cd2 + ,Cr6 + and Hg2 + .And the concentration ranges within which fluorescence was in-duced and with dose-response relationship were both in order of Cd2 + ,Hg2 + and Cr6 + .The de-tection limit of heavy metals was in order of Cr6 + ,Hg2 + and Cd2 + .However,the genetically modified Tetrahymena with GFP did not respond to copper.The results showed that the geneti-cally modified Tetrahymena with GFP can be used for detecting Cd2 + ,Hg2 + and Cr6 + pollution in environment.关键词
金属硫蛋白/绿色荧光蛋白/遗传改造四膜虫/重金属污染/荧光响应Key words
metallothionein/green fluorescent protein/genetically modified Tetrahymena/heavy metal pollution/florescence response分类
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梁海霞,刘红伟..GFP遗传改造四膜虫细胞的构建及其对重金属污染的荧光响应[J].太原理工大学学报,2017,48(6):929-933,945,6.基金项目
国家自然科学青年基金资助项目(31501124) （31501124）
山西省基础研究青年基金资助项目(201502119) （201502119）
