太原理工大学学报2017，Vol.48Issue(6)：1007-1013,7.DOI:10.16355/j.cnki.issn1007-9432tyut.2017.06.023
TNF-α和机械牵拉对圆锥角膜成纤维细胞MMP/TIMP及IL-6表达的影响
Effects of TNF-αand Mechanical Stretch on MMP/TIMP Ratios and the Expression of IL-6 in Keratoconus Corneal Fibroblasts
摘要
Abstract
Explored were the effects of tumor necrosis factor-α (TNF-α)and mechanical stretch on the expression of interleukin-6 (IL-6 ),matrix metalloproteinase-1,-3 (MMP-1, MMP-3),and tissue inhibitors of metalloproteinase-1,-2 (TIMP-1,TIMP-2)in keratoconus cor-neal fibroblasts.Isolated keratoconus corneal fibroblasts were subjected to 12% equibiaxial stretch at frequency of 0.1 Hz for 6 h in the presence of 1 ng/ml TNF-α.The gene expressions of IL-6,MMP-1,-3,and TIMP-1,-2 were detected by real-time polymerase chain reaction (Real-Time PCR).Culture supernatants were assayed by enzyme-linked immunosorbent assay (ELISA) for IL-6,MMP-1,-3,and TIMP-1,-2.TNF-α induced IL-6,MMP-1,MMP-3 mRNA expres-sion and protein synthesis (p <0.05),had no significant influence on the expression of TIMP-1,-2,and increased concentration ratios of MMP-1/TIMP-2 and MMP-3/TIMP-2 in keratoconus corneal fibroblasts (p <0.05);Cyclic stretch induced IL-6,MMP-1,MMP-3 mRNA expression and protein synthesis (p <0.05),inhibited expression of TIMP-1,-2 (p <0.05),and increased concentration ratios of MMP/TIMP in keratoconus corneal fibroblasts (p < 0.05 );TNF-α and stretch had a synergistic effect.Mechanical stretch combined with TNF-α may facilitate the cor-neal tissue destruction by upregulation of IL-6 and MMP/TIMP ratios,thereby contribute to the development of keratectasia.关键词
圆锥角膜/炎性因子/周期性牵拉/基质金属蛋白酶/基质金属蛋白酶抑制剂Key words
keratoconus/inflammatory cytokine/cyclic stretch/matrix metalloproteinases/tissue inhibitors of metalloproteinase分类
医药卫生引用本文复制引用
杜根来,李晓娜,陈维毅,刘成星,王晓君,贺瑞..TNF-α和机械牵拉对圆锥角膜成纤维细胞MMP/TIMP及IL-6表达的影响[J].太原理工大学学报,2017,48(6):1007-1013,7.基金项目
国家自然科学基金资助项目(31271005,11402161,11402162),山西省高等学校教学改革资助项目(J2012012) （31271005,11402161,11402162）
