中国组织工程研究2017，Vol.21Issue(28)：4475-4480,6.DOI:10.3969/j.issn.2095-4344.2017.28.008
新西兰大白兔距骨软骨细胞的分离培养与鉴定
Isolation, culture and identification of New Zealand white rabbit talar chondrocytes
摘要
Abstract
BACKGROUND:Based on the previous studies,the New Zealand rabbit talar chondrocyts were isolated,cultured and identified in vitro.OBJECTIVE:To explore the isolation,culture and identification of New Zealand rabbit talar chondrocyts in vitro.METHODS:The chondrocyts were isolated from the talar cartilage of New Zealand white rabbits by type Ⅱ collagen enzyme digestion,and then cultured in vitro.The cells were identified by inverted phase contrast microscope,toluidine blue staining and collagen type Ⅱ immunohistochemical staining.RESULTS AND CONCLUSION:Under the inverted phase contrast microscope,most of passaged chondrocytes presented with polygonal or triangle shape and had round or oval nuclei.Toluidine blue staining showed the hyacinthine chondrocytes and blue cellular matrix.Collagen type Ⅱ immunohistochemical staining showed that the chondrocytes appeared with brown granules in the cytoplasm and membrane.To conclude,a system that can isolate,culture and identify talar chondrocytes from New Zealand rabbits is successfully established.Talar chondrocytes at passages 1-3 grow well and have stable biological properties.关键词
组织构建/软骨细胞/距骨/兔/细胞分离/细胞培养/鉴定分类
医药卫生引用本文复制引用
王勇平,朱兆金,徐向阳..新西兰大白兔距骨软骨细胞的分离培养与鉴定[J].中国组织工程研究,2017,21(28):4475-4480,6.基金项目
兰州大学第一医院科研基金项(Idyyyn2017-21) （Idyyyn2017-21）
the Scientific Research Fund of the First Hospital of Lanzhou University,No.Idyyyn2017-21 （）
