中国组织工程研究2017，Vol.21Issue(32)：5115-5121,7.DOI:10.3969/j.issn.2095-4344.2017.32.006
创伤性脑损伤合并骨折中加速骨折愈合过程中相关因子的表达
Related factors accelerate fracture healing in traumatic brain injury combined with fracture
摘要
Abstract
BACKGROUND: Brain injury can promote fracture healing is becoming an issue of concern, but the underlying mechanism remains unknown.OBJECTIVE: To establish a model of cerebral injury combined with right tibial fracture, and to investigate the cerebrospinal fluid and serum levels of calcitonin gene-related peptide, nerve growth factor and insulin-like growth factor-1.METHODS: New Zealand white rabbits were randomly divided into four groups: group A: blank control group;group B: simple brain injury group; group C: simple tibial fracture group; group D: tibial fracture combined with brain injury. The cerebrospinal fluid and serum levels of calcitonin gene-related peptide, nerve growth factor and insulin-like growth factor-1 were detected at different time points. The rabbits in the groups C and D were sacrificed at 1, 4 and 8 weeks after modeling to remove the whole tibia, and scanned by X-ray. The callus volume was calculated, and the pathological changes were analyzed.RESULTS AND CONCLUSION: The fracture healing was faster in the group D than the group C. The callus volume,trabecular width, trabecular area ratio, and the number of newly born vessels in the group D were significantly higher than those in the group C at 4 weeks after modeling (P < 0.05). The cerebrospinal fluid and serum levels of calcitonin gene-related peptide, nerve growth factor and insulin-like growth factor-1 in the group D were significantly higher than those in the group C (P < 0.05), and the cerebrospinal fluid levels reached the maximum values earlier than did the serum levels. Furthermore, in the group D, the calcitonin gene-related peptide level was increased earlier than the other two factors. To conclude, calcitonin gene-related peptide, nerve growth factor and insulin-like growth factor-1 are essential factors involved in promoting fracture healing after traumatic brain injury, and moreover calcitonin gene-related peptide shows a stronger ability than the other factors.关键词
组织构建/骨组织工程/脑损伤/骨折愈合/降钙素基因相关肽/神经生长因子/胰岛素样生长因子1/加速/骨痂体积/血清/脑脊液/病理切片/异位骨化/新生血管/新疆维吾尔自治区自然科学基金分类
医药卫生引用本文复制引用
马乐园,赵岩,乔万庆,朱锋..创伤性脑损伤合并骨折中加速骨折愈合过程中相关因子的表达[J].中国组织工程研究,2017,21(32):5115-5121,7.基金项目
新疆维吾尔自治区自然科学基金(2014211C045) （2014211C045）
the Natural Science Foundation of Xinjiang Uygur Autonomous Region,No. 2014211C045 （）
