中国组织工程研究2017，Vol.21Issue(32)：5140-5145,6.DOI:10.3969/j.issn.2095-4344.2017.32.010
腰突颗粒调节腰椎间盘退变模型兔Fas/FasL基因的表达
Yaotu Granules regulate the Fas/FasL expression in a rabbit model of lumbar disc degeneration
摘要
Abstract
BACKGROUND: Yaotu Granules have been proved to protect human nucleus pulposus cells and delay their degeneration. Notably, Yaotu Granules for lumbar disc herniation has achieved good clinical results.OBJECTIVE: To investigate the effect of the herbal compound formula Yaotu Granules on the Fas/FasL expression in a rabbit model of lumbar disc degeneration, and further elucidate the underling mechanism of preventing and treating lumbar disc degeneration.METHODS: Twenty New Zealand white rabbits were enrolled and the models of lumbar disc degeneration were established by minimally invasive puncture and rotation cutting, followed by randomized into normal saline, low-, middle-,and high-dose groups (n=5 per group). 10 mL of normal saline, 10, 20, and 40 mL of water decoction of Yaotu Granules were administered intragastrically into the normal saline, low-, middle-, and high-dose drug groups for 21 days, twice daily, respectively. Subsequently, the expression level of Fas/FasL in the rabbit nucleus pulposus cells in each group was detected.RESULTS AND CONCLUSION: The signal intensity of the rabbit lumbar disc on MRI was decreased, and ruptured annulus and posterior herniated disc were visible at 12 weeks after modeling. Masson staining showed that the nucleus pulposus cells arranged in disorder, and even ruptured. Additionally, safranin O staining found that the number of nucleus pulposus cells was decreased obviously. The order of the relative expression levels of Fas and FasL mRNA in the nucleus pulposus cells was as follows: normal saline group > low-dose drug group > middle-dose drug group > high-dose drug group (P < 0.05). These results suggest that Yaotu Granules delay the rabbit lumbar disc degeneration by downregulating the expression level of Fas/FasL.关键词
组织构建/软骨组织工程/腰突颗粒/椎间盘退变/基因/Fas/FasL分类
医药卫生引用本文复制引用
何升华,赖居易,王业广,孙志涛,王建,冯华龙,黄飞强..腰突颗粒调节腰椎间盘退变模型兔Fas/FasL基因的表达[J].中国组织工程研究,2017,21(32):5140-5145,6.基金项目
深圳市科技计划项目(JCYJ20150401163247232) （JCYJ20150401163247232）
广东省科技厅(自筹项目)(2015[110号]) （自筹项目）
广东省中医药局项目(20162126) （20162126）
the Science and Technology Program of Shenzhen,No. JCYJ20150401163247232 （）
the Project of Guangdong Provincial Department of Science and Technology,No. 2015[110] （）
the Project of Traditional Chinese Medicine Bureau of Guangdong Province,No. 20162126 （）
