畜牧与兽医2017，Vol.49Issue(11)：61-68,8.
猪源伪狂犬病病毒AH02LA株糖蛋白的基因序列测定与对比分析
Sequencing analysis of glycoproteins gene of pseudorabies virus strain AH02LA
摘要
Abstract
Seventeen pairs of specific PCR primers were designed to amplify the fragments of 11 major envelope glycoprotein genes of a new pseudorabies virus (PRV) strain AH02LA,and the fragments were sequenced and analyzed.The alignment of the sequences was conducted with those of PRV mutant strains (TJ and HeN1) and PRV classical strains (Bartha and Kaplan) published in GenBank.The results showed that the 11 glycoprotein gene sequences of PRV AH02LA strain were successfully amplified and sequenced.Gene sequences alignment analysis showed that gB,gD,gG,gK and gM genes of AH02LA strain exhibited 100％ homology with the mutant strains,and 98％ to 99％ homology with the classical strains.And gC,gE and gI genes of AH02LA strain showed 99％ homology with the mutant strains,while 95％ to 97％ homology with the classical strains.Moreover,the insertion or deletion mutations found in gB,gC,gD,gE,gI and gN genes of AH02LA strain were the same as those in PRV mutant strains.It suggests that the genes encoding most glycoproteins of PRV AH02LA strain have high homology with PRV mutant strains isolated in China recently,indicating PRV AH02LA strain is an emerging mutant strain.关键词
伪狂犬病毒/AH02LA株/变异株/糖蛋白/序列分析Key words
Pseudorabies virus/AH02LA strain/variant/glycoproteins/sequence analysis分类
农业科技引用本文复制引用
许梦微,王义伟,乔永峰,王志胜,刘娅梅,侯继波,王继春..猪源伪狂犬病病毒AH02LA株糖蛋白的基因序列测定与对比分析[J].畜牧与兽医,2017,49(11):61-68,8.基金项目
公益性行业(农业)科研专项(201303046) （农业）
江苏省农业科技自主创新基金项目[CX (12) 3061 （12）
CX (14) 2084 （14）
] （）
江苏省自然科学基金项目(BK20131334) （BK20131334）
