畜牧与兽医2017，Vol.49Issue(11)：69-74,6.
犬瘟热病毒重组H蛋白间接ELISA检测方法的建立
Development of an indirect ELISA for detection of antibody against the recombinant H protein of canine distemper virus
摘要
Abstract
To establish an indirect ELISA method for detection of serum antibody to canine distemper virus (CDV),the main antigen fragment of HEMAGGLUTININ (H) gene was amplified from CDV genome and cloned into the pET-30a-c (+) vector.Then the recombinant H protein could be expressed in Rosetta (DE3) by IPTG induction.The results of SDS-PAGE and Western-blot analysis revealed that the recombinant H protein was successfully expressed and had good reactivity with the antiserum to CDV.Using the purified recombinant H protein as coating antigen,an indirect ELISA was developed for detection of antibody against CDV,and the ELISA method showed good results in specificity,sensitivity and reproducibility.The agreement rate of the indirect ELISA relative to commercial CDV antibody kit was above 92.5％ in detecting 80 mink sera.The indirect ELISA established in this study will provide a simple and rapid means for monitoring the antibody levels and evaluating the vaccine potency.关键词
犬瘟热病毒/原核表达/H蛋白/间接ELISAKey words
CDV/prokaryotic expression/H protein/indirect ELISA分类
农业科技引用本文复制引用
蔡孜萌,范威宏,张洪亮,姜合祥,单虎..犬瘟热病毒重组H蛋白间接ELISA检测方法的建立[J].畜牧与兽医,2017,49(11):69-74,6.基金项目
公益性行业(农业)科研专项(201303042) （农业）
