畜牧与兽医2017，Vol.49Issue(11)：96-100,5.
苏淮猪IFNGR1基因多态性及其与猪气喘病抗性的相关分析
Polymorphism analysis of IFNGR1 gene and its correlation with the resistance to Mycoplasmal pneumonia in Suhuai pigs
摘要
Abstract
Mycoplasmal pneumonia of swine is a chronic contagious respiratory disease and caused by Mycoplasma hyopneumoniae (Mhp).This disease causes huge economic losses to the pig industry.Based on the previous RNA sequencing results,we firstly selected out differentially expressed gene INFGR1 and identified the SNP of exons region in IFNGR1 gene by PCR amplification and sequencing of PCR products.And then the SNP was genotyped in Suhuai pig population from Huaiyin pig breeding farm in Huaian city.In addition,real-time fluorescence quantitative PCR was used to detect the infection level of Mhp in Suhuai pig population.Finally,the association analysis was performed between the SNP genotypes and Mhp infection level.Our results showed that a SNP (A12557G) in exon 5 was found.A allele frequency was 0.555 and the frequency of G allele was 0.445 in Suhuai pigs.The correlation analysis showed that the SNP genotype was highly significant with the infection level of Mhp in Suhuai pigs (P＜0.01) and the Mhp infection level in the pigs with genotype GG was higher than that in genotype AA.It suggested that IFNGR1 could serve as a promising genetic marker of breeding for resistance to mycoplasmal pneumonia in Suhuai pigs.关键词
苏淮猪/支原体肺炎/INFGR1/SNPKey words
Suhuai pig/mycoplasmal pneumonia/IFNGR1/SNP分类
农业科技引用本文复制引用
王士维,谢锐,段双丽,胡蒸蒸,李强,李平华,刘杨,黄瑞华..苏淮猪IFNGR1基因多态性及其与猪气喘病抗性的相关分析[J].畜牧与兽医,2017,49(11):96-100,5.基金项目
淮安市科技支撑计划(农业)产学研协同创新项目(HAC2015021) （农业）
江苏省农业科技自主创新项目(CX(15)1006) （CX(15）
江苏省自然科学基金项目(BK20140709) （BK20140709）
