医疗卫生装备2017，Vol.38Issue(11)：5-9,5.DOI:10.7687/j.issn1003-8868.2017.11.005
基于LabVIEW的细胞剪切力加载系统的设计及其实验验证
Development of LabVIEW-based loading system for cellular shear stress and its experimental validation
摘要
Abstract
Objective To develop a cellular shear stress loading system with an adjustable stress mode and relevant parameters, and subsequently verify the effectiveness and feasibility of this system. Methods The hardware of the system was developed by using a peristaltic pump and self-designed multi-channel flow chamber, and the mode control program of shear stress based on LabVIEW was designed to control the device via RS485 interfacing and Modbus protocol. Additionally, the relationship between the shear stress and system parameters was calibrated, and finite element analysis was also conducted. Finally, the feasibility of the system was confirmed via the in vitro cell experiment. Results The mode and magnitude of shear stress of the system could be controlled via either the peristaltic pump or computer, and the cellular long-term effect was also able to be detected. The calibration results of the system indicated that the level of shear stress exhibited significantly linear positive correlation with the revolution of the peristaltic pump (P<0.001). Finite element analysis demonstrated that the level of shear stress on the slide was uniformly distributed and the result of simulation was accordant with calibration. Cytoskeleton staining suggested that cellular morphology of MLO-Y4 cells was changed, and microfilament increased and arrayed along fluid flow direction. Conclusion The system is stable and reliable enough to provide different loading modes and magnitude of cellular shear stress to offer a convictive platform of the research for different cellular stress signal transduction mecha-nisms.关键词
剪切力/流体刺激/LabVIEW/程序控制/有限元分析Key words
shear stress/fluid flow stimulation/LabVIEW/program control/finite element analysis分类
医药卫生引用本文复制引用
颜泽栋,罗二平,王盼,刘梦婉,翟明明,张旭慧,刘曦雨,景达..基于LabVIEW的细胞剪切力加载系统的设计及其实验验证[J].医疗卫生装备,2017,38(11):5-9,5.基金项目
国家自然科学基金项目(31500760) （31500760）
