疑难病杂志2017，Vol.16Issue(11)：1132-1136,5.DOI:10.3969/j.issn.1671-6450.2017.11.014
二硫化二砷和靛玉红对骨髓增生异常综合征细胞MUTZ-1凋亡及其相关蛋白的影响
Effects of As2S2 and indirubin on apoptosis and related proteins of apoptosis in MUTZ-1 cell of myelodysplastic syndrome
摘要
Abstract
Objective To investigate the effects of As2S2 and indirubin of Qinghuangsan main ingredient on the cell cycle,apoptosis and apoptosis-related proteins Bcl-2,Bax and Caspase-3 in myelodysplastic syndromes (MDS) cell line MUTZ-1.Methods Experiment from June 2015 to June 2016 in China Academy of Chinese Medicine Xiyuan Hospital onset laboratories.With MDS-RAEB cell lines MUTZ-1 as the research object,the experiment including 4 groups:control group,group As2S2,indirubin group and combined group (As2S2 and indirubin joint application).To the As2S2,indirubin and two joint role respectively MUTZ-1 48 h and 72 h,cells by flow cytometry to detect the cell cycle,with Annexin V-FITC/PI method to detect cell apoptosis,with flow cytometry to detect apoptosis related proteins the Bcl-2,Bax and Caspase 3-expression.Results As2S2 and ndirubin for MUTZ-1 cells have obvious inhibitory effect.Compared with the control group,the As2S2 group and combined group at 48 h S phase and G2 / M phase cells to reduce,the proportion of G0/1 cell percentage increase (P ＜ 0.05);The percentage of S phase cells during 72 h,1 phase G0 / cells percentage increase (P ＜ 0.01),the proportion of G2 / M phase cells has no obvious changes (P =0.842).Group compared with control group,indirubin cells in different time points in each cell cycle has no obvious changes (P ＞ 0.05).And As2S2 compared two groups and indirubin group,a joint group of at 48 h S period and G2 / M phase cells proportion reduce G0/1 phase is increased (P ＜ 0.05) 72 h G0/1 phase proportion increases (P ＜0.01).Compared with control group,the As2S2 groups and indirubin early apoptosis cells increased significantly (P ＜ 0.05).With the As2S2 and indirubin group,united group to promote early apoptosis effect also enhanced (P ＜ 0.05).Compared with control group,indirubin group and combined group at 48 h cells express Caspase-3 increase (P ＜0.01),the As2S2 group at 72 h cells expressing the Bcl-2 cut (P ＜ 0.01),increased Bax (P ＜ 0.05).Compared with the As2S2 and indirubin group,combined group of MUTZ-1 cell the Bcl-2,Bax and Caspase-3 expression had no effect (P ＞ 0.05).Conclusion Qinghuangsan as main ingredient As2S2 and indirubin cells by promoting MDS MUTZ-1 apoptosis,influence of apoptosis related proteins play a role in the treatment of MDS,and use the As2S2 inhibits MUTZ-1 cell proliferation,and indirubin joint application can enhance the regulation of cell cycle and promote the role of cell apoptosis.关键词
二硫化二砷/靛玉红/骨髓增生异常综合征/MUTZ-1细胞/凋亡Key words
As2S2/Ndirubin/Myelodysplastic syndrome/MUTZ-1 cell/Apoptosis引用本文复制引用
杨秀鹏,周庆兵,麻柔,王洪志,胡晓梅,许勇钢..二硫化二砷和靛玉红对骨髓增生异常综合征细胞MUTZ-1凋亡及其相关蛋白的影响[J].疑难病杂志,2017,16(11):1132-1136,5.基金项目
国家自然科学基金资助项目(51803428) （51803428）
中国中医科学院自主选题研究项目(ZZ0708103) （ZZ0708103）
