医药导报2017，Vol.36Issue(12)：1350-1354,5.DOI:10.3870/j.issn.1004-0781.2017.12.004
少腹逐瘀汤对原发性痛经大鼠作用
Effect of Shaofu Zhuyu Decoction on Primary Dysmenorrhea in Rats
摘要
Abstract
Objective To investigate the effect of the Shaofu zhuyu decoction on the changes of the body writhing behavior,inflammatory reaction and COX-2 expression of the rats with the primary dysmenorrhea. Methods Fifty SD female rats were randomly divided into blank control group,model control group,high,middle and low dose of Shaofu zhuyu decoction groups,10 in each group.Dysmenorrhea rat model was established by treating with estradiol benzoate and oxytocin.Effect of Shaofu zhuyu decoction of different doses on writhing behavior,changes of endometritis cells and COX-2 expression in uterine smooth muscle of dysmenorrhea rats were observed. Results In the model control group,latency of the body writhing behavior was shortened and the total score was high,many inflammatory cells (especially for neutrophils) infiltrated in endometrium and uterine smooth muscle,immunohistochemistry showed that brown granules were found in the cytoplasm of smooth muscle cells of uterus,and COX-2 expression was positive in uterine smooth muscle cells.As compared with the model control group,writhing latency increased,total score decreased in both the high and middle dose of Shaofu zhuyu decoction groups,and infiltration of a small number of inflammatory cells was seen in the endometrium and smooth muscle(P<0.01);COX-2 expression was decreased (P<0.01) especially for the high dose of Shaofu zhuyu decoction group.As compared with the model control group,the latency of the low dose of Shaofu zhuyu decoction group was significantly increased,and the total score was decreased(P<0.01),but there was no significant difference in inflammatory cell infiltration and COX-2 expression (P>0.05). Conclusion A potential mechanism by which Shaofu zhuyu decotion treats primary dysmenorrhea may be related with alleviating pain,inhibiting inflammatory responses,and down-regulating expression of COX-2.关键词
少腹逐瘀汤/痛经,原发性/环氧化酶2/免疫组化Key words
Shaofu zhuyu decoction/Dysmenorrhea/primary/Cyclooxygenase-2/Immunohistochemistry分类
医药卫生引用本文复制引用
袁立明,周纤纤,杨梦洁,欧阳婉欣,贾诗杰,刘向光,毛豪初,陶晓军,杨华中..少腹逐瘀汤对原发性痛经大鼠作用[J].医药导报,2017,36(12):1350-1354,5.基金项目
湖南省自然科学基金面上项目(2016JJ2088) （2016JJ2088）
湖南省中药管理局科研项目(2015124) （2015124）
