诊断学理论与实践2017，Vol.16Issue(5)：504-509,6.DOI:10.16150/j.1671-2870.2017.05.010
Toll样受体4参与调控脂质诱导的平滑肌细胞炎症反应的研究
Toll-like receptor 4 regulates the inflammation induced by lipids in smooth muscle cells
摘要
Abstract
Objective:To investigate the mechanism of TLR4 in mediating the oxLDL-induced inflammation in smooth muscle cells (SMCs).Methods:TLR4 knockout mice and wild type C57BL/6 mice were randomly divided into control group (normal diet) and study group (high-fat diet) with 6 mice per group.Progression of atheroma was analyzed after 12 weeks in TLR4 knockout mice and wild type C57BL/6 mice.Primary SMCs of TLR4 knockout mice were cultured in vitro,and SMCs of wild type C57BL/6 were served as controls.SMCs were treated with oxLDL to induce inflammation,then TLR4 antibody was used to block TLR4 activity,and oxLDL-induced inflammation was compared between TLR4 knockout mice and wild type C57BL/6 mice.Results:High-fat diet could promote the expression of inflammatory cytokines in wild type C57BL/6 mice,but in TLR4 knockout mice there was no significant difference between the normal and high-fat diet groups.Moreover,blocking the activity of TLR4 could inhibit the oxLDL-induced inflammation in primary SMCs.Conclusions:TLR4 plays a role in regulating the oxLDL-induced inflammation in SMCs.关键词
Toll样受体4/平滑肌细胞,脂质/炎症反应/炎症因子Key words
Toll-Like Receptor 4/Smooth Muscle Cells/Lipid/Inflammation/Cytokines分类
医药卫生引用本文复制引用
王燕萍,陈媛媛,吴丽苹,陈亚芬,杨克,刘艳..Toll样受体4参与调控脂质诱导的平滑肌细胞炎症反应的研究[J].诊断学理论与实践,2017,16(5):504-509,6.基金项目
国家自然科学基金(81470547) （81470547）
上海市科委自然科学基金(15ZR1426100) （15ZR1426100）
