诊断学理论与实践2017，Vol.16Issue(5)：510-515,6.DOI:10.16150/j.1671-2870.2017.05.011
体外氟康唑诱导光滑念珠菌和近平滑念珠菌耐药及相关机制研究
Induction of fluconazole resistance in Candida glabrata and Candida parapsilosis in vitro and corresponding mechanism
摘要
Abstract
Objective:To investigate the susceptibility and mechanism of resistance to fluconazole in Candida glabrata and Candida parapsilosis isolates.Methods:Four strains (one clinical C.glabrata,one clinical C.parapsilosis,ATCC2001 and ATCC22019) were induced to be resistance strainsin vitro by increasing concentrations of fluconazole.The expression levels of transporter genes and their transcription factor,and azole antifungal target gene were identified by real time-PCR.Meanwhile,the sequence of PDR1 gene of C.glabrata and MRR1,ERGll gene of C.parapsilosis were determined by PCR based DNA sequencing.Results:C.glabrata was more prone to be resistant to fluconazole than C.parapsilosis.CDR1 was significantly overexpressed in resistant strains of C.glabrata,while MDR1 was significantly overexpressed in resistant strains of C.parwpsilosis.PDR1 mutations Y932C,V847F were identified in resistant strains of C.glabrata,and MRR1 mutations P295S,I799S were found in resistant strains of C.parapsilosis.Condusions:C.glabrata and C.parapsilosis showed different susceptibility to fluconazole resistance,and difference also occurred in resistance mechanism.And the function of new identified mutations in transcription factor needs further verification.关键词
念珠菌/氟康唑耐药/外排泵/靶酶/转录因子Key words
Candida spp/Resistance of fluconazole/Efflux pump/Target enzyme/Transcription factor分类
医药卫生引用本文复制引用
刘锦燕,王影,李文静,赵悦,孟玲宁,朱巍巍,史册,项明洁..体外氟康唑诱导光滑念珠菌和近平滑念珠菌耐药及相关机制研究[J].诊断学理论与实践,2017,16(5):510-515,6.基金项目
上海市黄浦区优秀青年人才(RCPY1407) （RCPY1407）
上海市医学重点专科(ZK2012A21) （ZK2012A21）
