中国病理生理杂志2017，Vol.33Issue(11)：2015-2019,5.DOI:10.3969/j.issn.1000-4718.2017.11.015
沉默Smo基因对人宫颈癌HeLa细胞活力及凋亡的影响
Effects of Smo gene silencing on cell activity and apoptosis of human cer-vical carcinoma HeLa cells
摘要
Abstract
AIM:To investigate the effect of Hedgehog(Hh) signaling pathway on the viability and apoptosis of cervical carcinoma cells by shRNA technique to knock down Smoothened (Smo) gene. METHODS:Smo shRNA was used to transfect the cervical carcinoma HeLa cells. The expression of Smo and Gli1 at mRNA and protein levels in the He-La cells was determined by RT-PCR and Western blot,respectively. The effect of Smo gene silencing on the growth of the cells was measured by MTT assay. The apoptosis and cell cycle were determined by flow cytometry. RESULTS:Compared with control group,the mRNA and protein expression of Smo and Gli1 were evenly reduced obviously after transfected with Smo shRNA for 72 h(P<0.05). The viability of HeLa cells transfected with Smo shRNA was significantly inhibited. The percentages of the cells in G0/G1phase and early apoptosis rate were obviously higher in Smo shRNA transfection group than those in control group. CONCLUSION:Smo gene silencing effectively inhibits the cell growth and induces the apop-tosis of human cervical carcinoma cells.关键词
Smo基因/宫颈癌/RNA干扰/细胞凋亡Key words
Smo gene/Cervical carcinoma/RNA interference/Apoptosis分类
医药卫生引用本文复制引用
宋凌,周强,李娜,余洁,李阳,张翅..沉默Smo基因对人宫颈癌HeLa细胞活力及凋亡的影响[J].中国病理生理杂志,2017,33(11):2015-2019,5.基金项目
商丘市科技发展计划(No.143008) （No.143008）
