中国病理生理杂志2017，Vol.33Issue(11)：2099-2102,2109,5.DOI:10.3969/j.issn.1000-4718.2017.11.029
雷公藤甲素在LPS诱导的急性葡萄膜炎中的抗炎作用
Anti-inflammatory effect of triptolide on LPS-induced uveitis
摘要
Abstract
AIM:To observe the anti-inflammatory effect of triptolide (TP) on lipopolysaccharide (LPS)-in-duced uveitis (LIU). METHODS:BALB/c mice (n=24) were randomly divided into 3 groups:blank control group (treated with normal saline), model group (treated with normal saline) and triptolide treatment group (treated with 0.05% triptolide eye drops),with 8 mice in each group. The mice in model group and triptolide treatment group were in-travitreally injected with LPS after 30 d of drug treatment. The anterior chamber was assessed by slit-lamp examination at different time points after modeling. Ocular tissues were collected for histological examination. The protein levels of inter-cellular adhesion molecule-1 (ICAM-1),interleukin-1β (IL-1β) and monocyte chemotactic protein-1 (MCP-1) in iridial and retinal samples were tested by ELISA. RESULTS:Compared with model group,the inflammatory reaction and clinical score were obviously decreased in triptolide treatment group at 24 h after modeling (P<0.01). The histopathological ob-servation indicated that infiltrating inflammatory cells were dramatically reduced in the anterior and posterior segments by triptolide eye drops. In addition, the expression levels of ICAM-1, IL-1β and MCP-1 were significantly decreased in the ocular tissues by treatment with triptolide (P<0.05). CONCLUSION:Triptolide prevents the injury of LIU by down-regulating inflammatory cytokines,and may be a new immune therapy for uveitis.关键词
雷公藤甲素/葡萄膜炎/炎症细胞因子Key words
Triptolide/Uveitis/Inflammatory cytokines分类
医药卫生引用本文复制引用
苏映雪,曹琛,甄栋钦,王延东..雷公藤甲素在LPS诱导的急性葡萄膜炎中的抗炎作用[J].中国病理生理杂志,2017,33(11):2099-2102,2109,5.基金项目
国家自然科学基金资助项目(No.81500739) （No.81500739）
广东省中医药局科研项目(No.20151168) （No.20151168）
广东省医学科学技术研究基金资助项目(No.A2015170) （No.A2015170）
