中国肺癌杂志2017，Vol.20Issue(11)：727-731,5.DOI:10.3779/j.issn.1009-3419.2017.11.01
利用高通量转录组测序对肺鳞癌基因型的研究
Study on Genetype in Lung Squamous Carcinoma by High-throughput of Study on Genetype in Lung Squamous Carcinoma by High-throughput of Transcriptome Sequence Transcriptome Sequence
摘要
Abstract
Background and objective Recently, there is no effective targeted drug for to the lung squamous carci-noma, the targeted drug for the adenocarcinoma does not benefit for the patients with squamous lung cancer, and researchers have known less targets for this type of patients. The main study herein is to screen and identify the specific key genes for the lung squamous carcinoma that will provide potential novel targets to describe pathogenesis of lung cancer.MethodsTran-script sequence was used to detect the five pairs lung squamous carcinoma and normal lung tissues and bioinformatics was per-formed to investigate the differential coding genes between them and quantitative PCR was done to validate the key genes ex-pression in lung cancer cell lines.Results The results of transcriptome sequence showed that 534 genes were up-regulated in the tumor tissues compared with the corresponding normal tissues. The top ten increased genes in the tumors wereGAGE12J, SPRR3,PRAME,SPRR1A,SPRR2E,MAGEA3,SPRR1B,IL36G andTMPRSS11D. Next, we identified expression of SPRR family in lung cancer cell lines H520, GLC82, A549, H1299 and PC9. And we found higher expression of SPRR family genes in H1299 cells which metastasized to the lymph node.ConclusionSPRR family genes are identified to be highly expressed in the lung squamous carcinoma by high throughput transcript sequencing. This SPRR family genes are associated with lymph node metastasis that provide novel idea for lung cancer therapy.关键词
肺肿瘤/高通量测序/SPRR家族Key words
Lung neoplasms/Transcriptome sequence/SPRR family引用本文复制引用
雄英,李明珍,张攀攀,张理意,杨跃..利用高通量转录组测序对肺鳞癌基因型的研究[J].中国肺癌杂志,2017,20(11):727-731,5.基金项目
本研究受北京大学"临床医院合作专项"以及北京市医馆局临床医学发展专项-"扬帆"计划(No.ZYLX201509)项目支持 This study was supported by the grants from Peking University (PKU) 985 Special Funding for Collaborative Research with PKU Hospitals and Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support (to Yue YANG)(No.ZYLX201509). （No.ZYLX201509）
