中国康复理论与实践2017，Vol.23Issue(11)：1263-1272,10.DOI:10.3969/j.issn.1006-9771.2017.11.006
脑源性神经营养因子预处理神经干细胞移植对急性缺血性脑卒中小鼠的效果
Effect of Neural Stem Cell Transplantation Pretreated with Brain-derived Neurotrophic Factor on Acute Isch-emic Stroke in Mice
摘要
Abstract
Objective To explore the effect of transplantation of neural stem cells(NSCs)pretreated with brain-derived neurotrophic fac-tor(BDNF)on acute ischemic stroke in mice.Methods NSCs from a newborn(one day)C57BL/6 mouse were isolated and cultured in vi-tro.A total of 150 healthy C57BL/6 mice,ten-week-old,were randomly divided into five groups,that group A accepted sham operation,and groups B,C,D and E were subjected to focal ischemia by photothrombosis.Group D was transplanted NSCs 24 hours after ischemia,while group E transplanted NSCs pretreated with BDNF and group C accepted same volume of medium.All the groups were tested with rotarod test and grip strength one day before transplantation and three,seven,14,21 and 28 days after transplantation.The differentiation of NSCs in groups D and E were observed immunofluorescence staining of microtubule-associated protein-2(MAP-2)and glial fibrillary acidic pro-tein(GFAP).Results The time on rotarod arranged from more to less was groups E,D and B(P<0.05)three,seven,14,21 and 28 days after transplantation,as well as grip strength 14,21 and 28 days after transplantation(P<0.05).The Edu/GFAP positive cells and Edu/MAP-2 pos-itive cells were found in both groups D and E.Conclusion Transplanting NSCs can promote the behavioral recovery after ischemic stroke, and it is more effective as pretreated with BDNF.关键词
缺血性脑卒中/神经干细胞/脑源性神经营养因子/体外培养/移植Key words
ischemic stroke/neural stem cells/brain-derived neurotrophic factor/in vitro/transplantation分类
医药卫生引用本文复制引用
王栋,杨文桢,侯博儒,康军林,任海军..脑源性神经营养因子预处理神经干细胞移植对急性缺血性脑卒中小鼠的效果[J].中国康复理论与实践,2017,23(11):1263-1272,10.基金项目
1.甘肃省高等学校科研项目(No.2014B-001) （No.2014B-001）
2.甘肃电信萃英科研基金资助项目(No.lzudxcy-2014-8). （No.lzudxcy-2014-8）
