中国康复理论与实践2017，Vol.23Issue(11)：1278-1283,6.DOI:10.3969/j.issn.1006-9771.2017.11.008
电针百会、大椎、肾俞穴对SAMP8小鼠学习记忆与海马CA1区神经元突触的影响
Effects of Electroacupuncture at Baihui,Dazhui and Shenshu Acupoints on Ability of Learning and Memory and Synapses of Neurons in Hippocampal CA1 in SAMP8 Mice
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) at Baihui (GV20), Dazhui (GV14) and Shenshu (BL23) acu-points on cognitive function and the synapse of neurons in hippocampal CA1 in SAMP8 mice,to explore the mechanism of EA in the treat-ment of Alzheimer's disease(AD).Methods A total of 24 seven-month-old SAMP8 mice were randomly divided into model group(n=12) and EA group (n=12), and the same age SAMR1 mice were as control group (n=12).The EA group accepted EA at Baihui, Dazhui and Shenshu for 30 days.They were assessed with Morris maze test.The expression of synaptophysin(SYN)and postsynaptic density protein 95(PSD95)in hippocampal CA1 region were detected with immunohistochemistry.The morphology and density of synapse in hippocampal CA1 region was observed with transmission electron microscopy.Results Compared with the model group,the latency of Morris maze de-creased in EA group(P<0.05),the time staying in the quadrant of the platform increased(P<0.05),as well as the number passing the origi-nal platform(P<0.05),with the more expression of SYN and PSD95 in hippocampal CA1 region(P<0.001),and more and completed syn-apse.Conclusion EA can improve the learning and memory ability of SAMP8 mice by increasing the expression of SYN and PSD95 to pro-tect the ultrastructure of synapses in hippocampal CA1 region.关键词
阿尔茨海默病/电针/学习记忆/突触素/突触后致密区蛋白95/小鼠Key words
Alzheimer's disease/electroacupuncture/learning and memory/synaptophysin/postsynaptic density protein 95/mice分类
医药卫生引用本文复制引用
李斐斐,杨珺,杨文丹,骆雍阳,陈慧娥,宋长明,董卫国..电针百会、大椎、肾俞穴对SAMP8小鼠学习记忆与海马CA1区神经元突触的影响[J].中国康复理论与实践,2017,23(11):1278-1283,6.基金项目
1.国家自然科学基金项目(No.81102625) （No.81102625）
2.福建省自然科学基金项目(No.2017J01541) （No.2017J01541）
3.福建省中医药科研项目(No.2017FJZYJC102) （No.2017FJZYJC102）
4.2015年度福建省高等学校新世纪优秀人才支持计划项目. （）
