中国康复理论与实践2017，Vol.23Issue(11)：1284-1289,6.DOI:10.3969/j.issn.1006-9771.2017.11.009
丁苯酞通过混合谱系激酶3信号通路对1-甲基-4-苯基-吡啶离子诱导的SH-SY5Y细胞增殖和凋亡的影响
Effects of DL-3-n-Butylphthalide on Proliferation and Apoptosis of 1-Methyl-4-Phenylpyridinium-induced SH-SY5Y Cells via Mixed Lineage Kinase 3 Signaling Pathway
摘要
Abstract
Objective To investigate the effects of DL-3-n-Butylphthalide(NBP)on proliferation and apoptosis of 1-methyl-4-phenyl-pyridinium (MPP +)-induced SH-SY5Y cells, and mechanisms via mixed lineage kinase 3 (MLK3) signaling pathway. Methods The SH-SY5Y cells were divided into control group,MPP+group,NBP group and URMC-099 group,that cultured normally,with 1 mmol/L MPP+for 24 hours,with 10μmol/L NBP for 3 hours and then with MPP+for 24 hours,and with 200 nmol/L MLK3 inhibitor URMC-099 for 3 hours and then with MPP+for 24 hours,respectively.The morphology of SH-SY5Y cells was observed under inverted phase contrast mi-croscope and the survival rate was measured with 3-(4,5-Cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assays.The apoptosis was quantified under flow cytometry with Annexin V/PI fluorescence staining,and the nuclear morphology was observed with Hoechst 33342 staining.The expression of phosphorylated protein of MLK3(p-MLK3),c-Jun N-terminal kinase(p-JNK),extra cellular regulated protein ki-nases(p-ERK1/2)were detected with Western blotting.Results Compared with the control group,the survival rate reduced and apoptosis in-creased in MPP+group(P<0.05),with the increase of p-MLK3 and p-JNK and decrease of p-ERK1/2 d(P<0.05).Compared with MPP+group,the survival rate increased and apoptosis reduced in both NBP and URMC-099 groups(P<0.05),with the decrease of p-MLK3 and p-JNK and increase of p-ERK1/2(P<0.05).Conclusion NBP can decrease the apoptosis and promote the proliferation of SH-SY5Y cells in-duced by MPP+,which may be associated with inhibiting MLK3 signaling pathway,and regulating the downstream p-JNK and p-ERK1/2.关键词
帕金森病/丁苯酞/混合谱系激酶3/凋亡/SH-SY5Y细胞Key words
Parkinson's disease/DL-3-n-butylphthalide/mixed-lineage kinase 3/apoptosis/SH-SY5Y cells分类
医药卫生引用本文复制引用
郭子梦,吴庆文,陈秀秀,关亚丽,李鹏飞,王妍,程月发..丁苯酞通过混合谱系激酶3信号通路对1-甲基-4-苯基-吡啶离子诱导的SH-SY5Y细胞增殖和凋亡的影响[J].中国康复理论与实践,2017,23(11):1284-1289,6.基金项目
1.华北理工大学研究生创新项目(No.2017S37) （No.2017S37）
2.华北理工大学博士科研启动基金项目(No.35647699) （No.35647699）
3.河北省高等学校科学研究青年基金项目(No.QN201722). （No.QN201722）
