中国免疫学杂志2017，Vol.33Issue(11)：1652-1657,6.DOI:10.3969/j.issn.1000-484X.2017.11.011
脊髓康对共培养体系中小胶质细胞吞噬及损伤神经元再生的影响
Effects of Chinese herbal compound "Jisuikang" on phagocytosis of microglia and regeneration of injured neurons in co-culture system
摘要
Abstract
Objective:To observe the effects of Chinese herbal compound'Jisuikang'on the phagocytosis of microglia and the regeneration of injured neurons in co-culture system.Methods: Prepared drug serum of 'Jisuikang′ and isolated and identified the primary neuron and microglia.The neuron cells were induced apoptosis by glutamic acid and the microglia cells were predisposed by drug serum of'Jisuikang'.Then,the co-culture system of injured neurons and microglia cells was established.24 h and 96 h after co-culture,engulfment of neuron debris by microglia cells and regeneration of injured neurons were observed by immunofluorescence double labeling method.Results: 24 h after co-culture,middle and high dose of'Jisuikang' showed greater phagocytic percentage and phagocytic index than that of control.In comparison of LPS,high dose of'Jisuikang' showed no significant difference.96 h after co-culture,first grade of neuritis of middle and high dose of'Jisuikang' were more than that of control,and there were no significant difference in comparison of LPS.Neuritis' mean length per cell of middle and high dose of'Jisuikang' were larger than that of con-trol.Neuritis' mean length per cell of high dose of'Jisuikang' showed significant difference in comparison of LPS.Conclusion:Traditional Chinese medicine compound'Jisuikang'may enhance engulfment of neuron debris by microglia to improve local microenvi-ronment,which promote the repair and regeneration of injured neurons.关键词
中药复方/脊髓康/原代小胶质细胞/原代神经元/共培养/吞噬作用/神经元修复再生Key words
Herbal compound/Jisuikang/Primary microglia/Primary neurons/Co-culture/Phagocytosis/Regeneration of neu-rons分类
医药卫生引用本文复制引用
潘娅岚,郭杨,周龙云,苑文超,马勇,黄桂成..脊髓康对共培养体系中小胶质细胞吞噬及损伤神经元再生的影响[J].中国免疫学杂志,2017,33(11):1652-1657,6.基金项目
本文受国家自然科学基金青年项目(81704100)、国家自然科学基金面上项目(81573997)和江苏省高校自然科学研究面上项目(15KJD360001)资助. （81704100）
