中国免疫学杂志2017，Vol.33Issue(11)：1678-1681,4.DOI:10.3969/j.issn.1000-484X.2017.11.016
自身免疫性脑炎患者外周血Tfh细胞的检测及其临床意义
Detection and clinical significance of peripheral blood follicular helper T cells in autoimmune encephalitis patients
摘要
Abstract
Objective:To investigate the proportion of follicular helper T cells(Tfh) and its clinical significance in peripheral blood of patients with autoimmune encephalitis(AE).Methods:①Cerebrospinal fluid(CSF) specimen was collected from the patients who were confirmed to have autoantibodies associated with autoimmune encephalitis,and the CSF was incubated with frozen sections of rat brain tissue,and the immunofluorescence staining was performed. ②17 patients were collected (3 cases with anti LGI1 antibody positive,3 cases with anti GABAR antibody positive,11 cases with anti NMDAR antibody positive, and 1 case with anti NMDAR antibody,anti GABAR antibody and anti AMPA2 antibody positive).All of the above,21 samples were collected with peripheral blood:6 cases with no therapy;15 cases with therapy(contains 8 remission ones and 7 no remission ones).15 healthy controls(control group) were collected. The percentage of CD3+CD4+CXCR5+PD1+T cells were detected by flow cytometry (FCM).The correlation between the Tfh cells and the disease recovery was also analyzed.Results:①Cerebrospinal fluid of patients incubated with frozen sections of rat brain tissue,immunofluorescence staining results showed positive reaction.②The flow cytometry results showed that the percentage of peripheral blood Tfh cells in patients with untreated group was significantly higher than that in the control group(P<0.001),and after treatment the percentage of Tfh in no improved group was higher than improved group(P<0.05).But the percentage of Tfh cells had no statistical difference between the improved group and the control group(P=0.107).Conclusion:The increase of peripheral blood Tfh cells percentage in the AE patients may contribute to the occurrence and development of AE.关键词
自身免疫性脑炎/滤泡辅助性T细胞/自身抗体Key words
Autoimmune encephalitis/Follicular helper T cells/Autoantibody分类
医药卫生引用本文复制引用
刘婷婷,杜芳,孙元杰,杨毅宁,代文,赵钢,杨琨..自身免疫性脑炎患者外周血Tfh细胞的检测及其临床意义[J].中国免疫学杂志,2017,33(11):1678-1681,4.基金项目
本文受国家自然科学基金面上项目(No.81171977)资助. （No.81171977）
