中国农业大学学报2017，Vol.22Issue(11)：17-27,11.DOI:10.11841/j.issn.1007-4333.2017.11.02
红麻不育系与保持系基因组DNA甲基化比较分析
Comparative analysis on the kenaf (Hibiscus cannabinus L.) genomic DNA methylation of its male sterility line and maintainer line
摘要
Abstract
Taking the CMS line UG93A and maintainer line UG93B of kenaf as materials,gDNA(genomic DNA) were extracted from seedling leaves,tetrad stage anthers and binucleate stage anthers respectively.MSAP (methylation sensitive amplified polymorphism) technology was used to analyze and compare their methylation of gDNA.The expression patterns of methylated genes were studied by qRT-PCR.The results showed that:1) During the growth and development of kenaf,DNA methylation showed spatio-temporal variation pattern.The gDNA methylation rate (MSAP％) of UG93A and UG93B at seedling stage were 56.79％ (full methylated rate was 44.25％,similar as follows) and 58.89 ％ (43.24 ％);MSAP％ at tetrad stage was 48.08 ％ (36.24 ％) and 44.25 ％ (33.22 ％);MSAP％at binucleate stage was 45.30％ (34.15％) and 48.78％ (37.98％),respectively.2) The ratio of UG93A was the highest at seedling stage,tetrad stage before anther abortion came the second,then binucleate stage after abortion.It displayed a trend of high to low during growth and development.The rate of UG93B was highest at seedling stage,followed by binucleate stage and tetrad stage showing a trend of high to low,and then gradually rising.Although,the rate of UG93B was higher than that of UG93A,but the UG93A was significantly higher than UG93B in the tetrad stage before anther abortion.3) Gene methylation or demethylation happened in many genes including atp8、SCL13、SRF6、phytosulfokine receptor 2.These genes in UG93A and UG93B were differentially methylated.The results from qRT-PCR analysis reviewed that the differentially expression levels of methylated fragments between the cytoplasmic male sterile line and maintainer line showed significant differences,indicating gene methylation played an important role of in kenaf CMS.关键词
红麻/基因组DNA甲基化/CMS/MSAP/qRT-PCRKey words
kenaf/DNA methylation/CMS/MSAP/qRT-PCR分类
农业科技引用本文复制引用
李增强,陈鹏,史奇奇,孔祥军,汤丹峰,廖小芳,韦范,何冰,莫良玉,周瑞阳..红麻不育系与保持系基因组DNA甲基化比较分析[J].中国农业大学学报,2017,22(11):17-27,11.基金项目
国家自然科学基金(31260341,31560421) （31260341,31560421）
广西自然科学基金(2015GXNSFAA139059) （2015GXNSFAA139059）
国家现代农业产业技术体系(CARS-19-E16) （CARS-19-E16）
