中国农业大学学报2017，Vol.22Issue(11)：114-120,7.DOI:10.11841/j.issn.1007-4333.2017.11.13
β-胡萝卜素的不同添加方式对脂多糖刺激的RAW264.7细胞炎症的影响
Effects of different β-carotene adding models on the inflammation of LPS-induced RAW264.7 cells
摘要
Abstract
The aim of this study was to investigate the effects of β-carotene treatment model and prevention model on cell viability,ROS,inflammatory factors and NF-κB p65 protein in LPS-induced RAW264.7 cells.To determine the most suitable concentration and time of β-carotene,the cell viability in two models was measured by MTT.The percentage of ROS was detected by flow cytometry analysis,and the mRNA relative expressions of IL-1β,IL-6 and TNF-α were measured by fluorescence quantitative PCR.The protein relative expressions of NF-κB p65 were measured by Western Blot to research the effect of β-carotene on NF-κB signaling pathway.The results showed that:In LPS-induced RAW264.7 cells,the cell viabilities of treatment model and prevention model were higher and the difference was very significant in treatment model (P＜O.01);The percentage of ROS,expression of inflammatory factors and protein NF-κB p65 were lower than LPS-induced group.In summary,β-carotene promoted the cell viability of LPS-induced RAW264.7 cells and inhibited the percentage of ROS and expression of inflammatory factors,indicating the correlation of the inhibition effect with NF-κB pathway.关键词
B-胡萝卜素/RAW264.7细胞/细胞活力/活性氧/炎性因子Key words
β-carotene/RAW264.7 cell/cell viability/ROS/inflammatory factor分类
农业科技引用本文复制引用
张晓音,吴旻,张珊珊,吉昱斌,黄羽盛,郑鑫..β-胡萝卜素的不同添加方式对脂多糖刺激的RAW264.7细胞炎症的影响[J].中国农业大学学报,2017,22(11):114-120,7.基金项目
国家自然科学基金面上项目(31672511) （31672511）
