中国农业大学学报2017，Vol.22Issue(12)：53-63,11.DOI:10.11841/j.issn.1007-4333.2017.12.07
芍药内外花瓣发育形成相关基因片段的生物信息学及组织表达分析
Bioinformatics and tissue expression analysis of genes affecting the formation and development of inner/outer petal in Paeonia lactiflora
摘要
Abstract
To reveal the molecular mechanisms of petal formation in Paeonia lactiflora,we selected inner and outer petals from P.lactiflora'Zi Fengyu'with anemone type as study object.Using transcriptome sequencing,key gene sequences regulating flower type development were screened.The protein structure and function,subcellular localization were firstly analyzed by bioinformatics method,and then the expression level of petals detected by qPCR.The results showed that two key genes,namely AGAMOUS (AG) and MADS-box protein 2 (MADS2),were determined by sequencing.The protein analysis displayed that AG and MADS2 protein were liposoluble,hydrophilic and unstable.No signal peptide and transmembrane structure indicated two proteins were non-secretory,α-helices were its main elements of the secondary structure and their three-dimensional structure were very similar.Besides,AG and MADS2 protein contained same functional MADS domain.These proteins mostly distributed in other locations rather than in signal peptide secretion pathway,qPCR analysis showed the expression of MADS2 and AG in inner-petal was extremely significant than that in outer-petal.The similarity of AG and MADS2 in protein structure,function and expression suggested that the two proteins probably played same biological functions in the formation process of P.lactiflora petal.关键词
芍药/托桂型/生物信息学/基因表达Key words
Paeonia lactiflora/flower type/bioinformatics/gene expression分类
农业科技引用本文复制引用
吴彦庆,汤寓涵,赵大球,陶俊..芍药内外花瓣发育形成相关基因片段的生物信息学及组织表达分析[J].中国农业大学学报,2017,22(12):53-63,11.基金项目
国家自然科学基金(31372097 （）
31400592) （）
江苏省高校自然科学研究重大项目(13KJA210005) （13KJA210005）
