中国农业大学学报2017，Vol.22Issue(12)：64-73,10.DOI:10.11841/j.issn.1007-4333.2017.12.08
枸杞苯丙氨酸解氨酶基因的克隆与表达分析
Molecular cloning and expression patterns of LcPAL from Lycium chinense
摘要
Abstract
To investigate the role of LcPAL from Lycium chinense in drought and salt stress,a phenylalanine ammonialyase(PAL)gene was cloned from Lycium chinense by the methods of reverse-transcription PCR and RACE.The fulllength cDNA of L.chinense PAL (designated as LcPAL)was 2 544 bp(Genbank No.KX781247)containing a complete open reading frame(ORF)of 2 163 bp,which encoded 720 amino acid residues.Sequence analysis showed that it had typical PAL enzyme active site sequence(GTITASGDLVPLSYIA).Homology analysis indicated that the deduced LcPAL protein was highly homology to other PAL proteins from different species.Phylogenetic tree analysis revealed that LcPAL had closer relationship with PALs from Solanaceae plants than from other plants.The results from Real-Time PCR indicated that the epression of LcPAL gene was the strongest in the leaves of L.chinense,and the least in fruits.Furthermore,LcPAL transcription level was induced under the treatment of PEG and NaCl stresses,but the expression patterns were different.As a result,the gene LcPAL which was cloned and characterized from L.chinense is a member of PAL family typically.The result of study indicated that LcPAL played a role in the development of L.chinense plant.LcPAL also played a role in resistance to drought and salt stress processes.关键词
枸杞/苯丙氨酸解氨酶(PAL)/基因克隆/表达分析Key words
Lycium chinense/phenylalanine ammonia-lyase (PAL)/gene cloning/expression analysis分类
生物科学引用本文复制引用
乔枫,耿贵工,张丽,金兰,陈志..枸杞苯丙氨酸解氨酶基因的克隆与表达分析[J].中国农业大学学报,2017,22(12):64-73,10.基金项目
青海省科技厅项目(2015-ZJ-735) （2015-ZJ-735）
教育部项目(Z2016098) （Z2016098）
青海省科技厅重点项目(2017-zj-y13) （2017-zj-y13）
