中国农业大学学报2017，Vol.22Issue(12)：95-100,6.DOI:10.11841/j.issn.1007-4333.2017.12.11
梅花鹿Ghrelin全长cDNA克隆及其序列分析
Full-length cDNA sequence cloning and analysis of Ghrelin in Cervus nippon
摘要
Abstract
In order to obtain the full-length cDNA of Ghrelin in Cervus nippon,RT-PCR and RACE methods were used by using total RNA of abomasus tissue in C.nippon as template.The results of sequence analysis revealed a 539 bp length cDNA containing 46 bp 5'-untranslated region (5'UTR),128 bp 3'-untranslated region (3'UTR) and 351 bp open reading frame (ORF) encoding 116 amino acids.The cDNA sequence alignments of C.nippon Ghrelin gene with human and other animals showed that the cDNA sequence homology of C.nippon Ghrelin was 90.4％-99.1％ to reindeer,goat,sheep and cattle,66.9％-76.6％ with rhesus monkey,human,pig and dog,only 36.4％ with chicken and C.livia.These results indicated that the structure of Ghrelin displayed an obvious varietal specificity,suggesting that Ghrelin might play an important physiological function role in ruminants.关键词
梅花鹿/Ghrelin/cDNA克隆/同源性分析Key words
Cervus nippon/Ghrelin/cDNA clone/homology analysis分类
农业科技引用本文复制引用
张曼,金鑫,田巧珍,刘骄,王云鹤,杨银凤..梅花鹿Ghrelin全长cDNA克隆及其序列分析[J].中国农业大学学报,2017,22(12):95-100,6.基金项目
内蒙古自然科学基金资助项目(2013MS0408) （2013MS0408）
