中国全科医学2017，Vol.20Issue(26)：3246-3250,5.DOI:10.3969/j.issn.1007-9572.2017.04.y01
亚砷酸钠中毒对大鼠海马组织神经元及肾功能的影响研究
Impact of NaAsO2 Poisoning on Rat Hippocampal Neurons and the Kidney Function
摘要
Abstract
Objective To observe the changes in histology and morphology of hippocampal CA3 region neurons and renal tissues in adult SD rats after NaAsO2 poisoning.Methods This study was conducted from February to June in 2016.Forty -eight male SD rats (SPF grade) aged 12 weeks were randomly assigned to the normal control group,low-,medium-and high-dose NaAsO22 infected groups with 12 in each,respectively treated by freely drinking of distilled water,5,10,20 mg/kg NaAsO2 solution for an intervention of 12 weeks.All the rats were sacrificed and the hippocampal and kidney tissues were taken at the end of intervention.Light microscope was used to observe the morphology changes in hippocampal and renal tissues stained by HE.The activity of superoxide dismutase (SOD) and concentrations of reduced glutathione (GSH) and malondialdehyde (MDA) in these tissues were determined.Results The observation showed that,compared with the control group,in hippocampal region of the NaAsO2 infected groups,the nerve cells were irregular with decreased number and enlarged interspaces,moreover,a large number of exfoliated and necrotic nerve cells could be seen;pathological changes were found in the renal tissues,including degeneration,necrosis,edema and inflammatory cell infiltration.The activity of SOD and concentrations of GSH and MDA in hippocampal and renal tissues differed significantly among the four groups (P ＜ 0.05),specifically,the NaAsO2 infected groups had lower activity of SOD and concentration of GSH but higher concentration of MDA in hippocampal and renal tissues than the control group did;the medium-and high-dose NaAsO2 infected groups had lower activity of SOD and concentration of GSH but higher concentration of MDA in hippocampal and renal tissues than the low-dose NaAsO2 infected group did;the high-dose NaAsO2 infected group had lower activity of SOD and concentration of GSH but higher concentration of MDA in hippocampal and renal tissues than the medium-dose NaAsO2 infected group did (P ＜ 0.05).Conclusion NaAsO2 poisoning can cause rat hippocampal neurons morphology and renal tissue structure damage,and oxidative stress may be involved in the process.关键词
砷中毒/CA3区,海马/肾功能/氧化应激Key words
Arsenic poisoning/CA3 region,hippocampal/Kidney function/Oxidative stress分类
医药卫生引用本文复制引用
秦文娟,管东方,邢国强,史红娟,吕海龙,姜玉峰..亚砷酸钠中毒对大鼠海马组织神经元及肾功能的影响研究[J].中国全科医学,2017,20(26):3246-3250,5.基金项目
国家自然科学基金资助项目(81360410) （81360410）
