中国人兽共患病学报2017，Vol.33Issue(10)：868-871,4.DOI:10.3969/j.issn.1002-2694.2017.10.003
空肠弯曲菌AhpC蛋白B细胞抗原表位预测及抗原性分析
Campylobacter jejuni AhpC protein B cell antigen epitope prediction and antigenic analysis
摘要
Abstract
To provide evidence for Campylobacter jejuni (C.jejuni) vaccine research,the B cell epitope of AhpC protein were predicted,and the antigenicity was analyzed.AhpC was found locating in outer membrane of C.jejuni without transmem brane structure and no signal peptide by bioinformatics software TMHMM Server V2.0,SignalP 4.1.There were seven B-cell linear epitopes in AhpC predicted by IEDB.Then,the AhpC protein and chemically synthesized antigenic epitopes of C.jejuni were used as antigens,and the AhpC antibody of C.jejuni was used as the primary antibody,the antigens of predominant linear B cell epitopes were detected by ELISA and dot blot.Results showed that one epitope of B cell epitopes (AhpC4-16) was able to recognize the antibodies of C.jejuni AhpC and had strong antigenicity,indicating that could be used in the follow-up vaccine research.关键词
空肠弯曲菌/表位/抗原/烷基过氧化氢还原酶Key words
Campylobacter jejuni/epitope/antigen/alkyl hydroperoxide reductase引用本文复制引用
陈浩浩,李旭升,郭方明,丁明星,胡野,楼宏强..空肠弯曲菌AhpC蛋白B细胞抗原表位预测及抗原性分析[J].中国人兽共患病学报,2017,33(10):868-871,4.基金项目
Supported by grants from the Science and Technology Planning Project of Zhejiang Province (Nos.2016C33229,2016C37093),the Science and Technology Planning Project of Jinhua City (No.2016-3-002).浙江省科技计划项目(No.2016C33229),浙江省科技计划项目(No.2016C37093)和金华市科技研究计划项目(No.2016-3-002)联合资助 （Nos.2016C33229,2016C37093）
