中国人兽共患病学报2017,Vol.33Issue(10):872-876,5.DOI:10.3969/j.issn.1002-2694.2017.10.004
福建省登革热监测点白纹伊蚊mtDNA-COI基因遗传多态性研究
Genetic diversity of cytochrome C oxidase subunit Ⅰ genes in Aedes albopictus from dengue surveillance sites in Fujian Province, China
摘要
Abstract
The mosquito Aedes albopictus is the primary vector for dengue virus transmission in Fujian Province.Despite that active dengue surveillance has been launched in several sites since 2005,the genetic diversity of A.albopictus from these sites remains exclusive.In this study,mosquito pools collected from dengue surveillance sites during 2015-2016 were randomly selected,the full-length mitochondrial cytochrome C oxidase subunit Ⅰ (mtDNA-COⅠ) were amplified and sequenced.Preliminary sequence alignment of 12 amplicons with the reference sequence indicated 99 % homology at nucleotide level,due to varia tions at 9 nucleotide sites.Three haplotypes,namely H02,H03 and H08,were determined based on phylogenetic analysis with 72 reference sequences of known haplotypes.These observations facilitate surveillance and control of arboviral diseases in Fujian.关键词
白纹伊蚊/线粒体细胞色素C氧化酶亚基Ⅰ/遗传多态性/种系发生分析Key words
Aedes albopitus/mitochondrial cytochrome C oxidase subunit Ⅰ/genetic diversity/phylogenetic analysis引用本文复制引用
游丽斌,朱颖,何文祥,翁育伟,王金章,阚乃鹏,张拥军..福建省登革热监测点白纹伊蚊mtDNA-COI基因遗传多态性研究[J].中国人兽共患病学报,2017,33(10):872-876,5.基金项目
Supported by the Department of Science and Technology,Fujian Province (No.2016Y0011) and the Innovative Project of Medical Science of Fujian Province (No.2015-CXB-13).福建省科技厅引导性项目(No.2016Y0011)和福建省医学创新课题(No.2015-CXB-13)联合资助 (No.2016Y0011)