中国人兽共患病学报2017，Vol.33Issue(10)：888-892,902,6.DOI:10.3969/j.issn.1002-2694.2017.10.007
医源与动物源肺炎克雷伯菌耐药性和分子特性研究
Drug-resistant and molecular characteristic of Klebsiella pneumoniae isolated from nosocomial and animal origins
摘要
Abstract
We aimed to detect the drug resistant and molecular characteristic of Klebsiella Pneumoniae (KP) isolated from different hosts origin,and to investigate the infection possibility between animals and humans.A total of 98 KP strains were collected in Henan Province from March 2013 to December 2014.Drug-resistance to 15 antibiotics was detected by K-B diffu sion methods.The phenotype of produce mucus was determined by stringing test.Seven drug-resistant gene and 2 virulence gene were amplified by PCR technique.Molecular types were analysis by MLST.The resistant rate of KP isolated from noso comial was higher than those isolated from animals.Among animal strains,the resistant rate of KP isolated from chickens and pigs were higher than that from rabbits and dogs.The multidrug resistant (MDR) of nosocomial isolates were the highest (74.19％).There were 18 STs among 98 KP strains.The main prevalent types were ST37 in chicken,ST258 in pigs,ST60 in rabbits,ST11 in dogs and nosocomial respectively.ST11 was common epidemic types among nosocomial,dogs and pigs.ST235 was common molecular types among chicken and human.ST258 existed in both nosocomial and pigs.The rmpA gene and magA gene were detected in ST11,ST235 and ST 258,producing higher mucus.The blaKPC gene was 54.84％ in nosocomial strains,but not been detected in dog and rabbit strains.The distribution of ESBLs gene in nosocomial was higher than those in animals,but qnrA gene and qnrB gene were higher in chicken than in human.The multidrug resistant gene was the highest distribution in ST11,ST258 and ST235.There were some differences in phenotype of drug-resistance,producing mucus and molecular characteristics,but ST11,ST258 and ST235 were common types of KP isolates from different host origins.关键词
肺炎克雷伯菌/耐药性/MLST/医源/动物源Key words
Klebsiella pneumoniae/drug-resistant/MLST/nosocomial/animal origin分类
医药卫生引用本文复制引用
杨帆,邓保国,魏纪东,赵永新,李敏..医源与动物源肺炎克雷伯菌耐药性和分子特性研究[J].中国人兽共患病学报,2017,33(10):888-892,902,6.基金项目
河南省高等学校青年骨干教师资助项目(No.2015GGJS-134).Supported by the University Youth Backbone Teachers Projects of Henan Province (No.2015GGJS-134). （No.2015GGJS-134）
